Simplifying minicircle production
Optimized for minicircle production, the ZYCY10P3S2T Minicircle Production Strain comes as competent cells, so you can directly transform ligation reactions or plasmids. The competent cells are prepared and tested using a modification of the procedure developed by Kay, et al.1, and are suitable for cloning and production of minicircle-based plasmids.
The ZYCY10P3S2T E.coli Minicircle Production Strain is derived from the BW27783 E. coli strain that stably expresses a set of inducible minicircle-assembly enzymes, PhiC31 integrase, and I-SceI homing endonuclease. This bacterial strain enables production and purification of minicircles in a time frame and quantity similar to that of routine plasmid DNA preparation.
Please note that other competent E. coli strains cannot be substituted for minicircle production as they do not have the appropriate genomic modifications to produce minicircles.
About Minicircle Technology
- Episomal expression sustained over weeks
- Foreign DNA-free
- More efficient transfections from small plasmid size
- Unlimited insert size
- Optimized coli minicircle production strain
- Works in vitro and in vivo
When you want sustained transgene expression without introducing any foreign DNA—such as for model animal and gene therapy development—Minicircle Technology is a great gene expression option. Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.
Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle DNA production to both not-for-profit and commercial end users—contact firstname.lastname@example.org for additional details. For any other purposes, including the ability to buy the parental MC production system, commercial users should contact SBI at email@example.com for further information.
- Kay MA, He CY, and Chen ZY. A robust system for production of minicircle DNA vectors. Nat Biotechnol. 2010 Dec; 28(12):1287-9. PMCID: PMC4144359.