pCDH-UbC-MCS-EF1α-Hygro Cloning and Expression Lentivector

Use the pCDH-UbC-MCS-EF1α-Hygro when you want low expression of your gene of interest with a hygromycin resistance marker

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pCDH-UbC-MCS-EF1α-Hygro cDNA Dual Promoter Cloning and Expression Lentivector

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pCDH-UbC-MCS-EF1α-Hygro cDNA Dual Promoter Cloning and Expression Lentivector

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10 µg
CD615B-1
$ 515
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Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for high, reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-UbC-MCS-EF1α-Hygro Cloning and Expression Lentivector drives expression of your gene-of-interest from the low-expression UbC promoter, and expression of the hygromycin resistance marker from an EF1α promoter.

pCDH-UbC-MCS-EF1α-Hygro Cloning and Expression Lentivector

With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:

  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Not sure which promoter is right for you? This table should help:

Promoter Expression Level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells


Citations

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  • Li, X, et al. (2017) MicroRNA‑146a‑5p enhances cisplatin‑induced apoptosis in ovarian cancer cells by targeting multiple anti‑apoptotic genes. Int. J. Oncol.. 2017 Jul 1; 51(1):327-335. PM ID: 28560455
  • Zhou, F, et al. (2017) AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia. Nat. Cell Biol.. 2017 Jul 1; 19(7):844-855. PM ID: 28650479
  • Li, SH, et al. (2017) Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer’s Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice. Front Neurosci. 2017 Jun 29; 11:340. PM ID: 28659755
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  • Gu, Z, et al. (2017) Increased PTPRA expression leads to poor prognosis through c-Src activation and G1 phase progression in squamous cell lung cancer. Int. J. Oncol.. 2017 Jun 23;. PM ID: 28656243
  • Franzetti, GA, et al. (2017) Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells. Oncogene. 2017 Jun 22; 36(25):3505-3514. PM ID: 28135250
  • Katayama, M, et al. (2017) Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J. Reprod. Dev.. 2017 Jun 21; 63(3):311-318. PM ID: 28331164
  • Guo, Y, et al. (2017) Gelsolin regulates proliferation, apoptosis and invasion in NK/T-cell lymphoma. biorxiv.org. 2017 Jun 20;. Link: biorxiv.org
  • Al-Khalaf, HH, et al. (2017) Obesity and p16(INK4A) down-regulation activate breast adipocytes and promote their pro-tumorigenicity. Mol. Cell. Biol.. 2017 Jun 19;. PM ID: 28630279
  • Ye, L, et al. (2017) miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2. Mol Ther Nucleic Acids. 2017 Jun 16; 7:453-464. PM ID: 28624221
  • Cicchini, L, et al. (2017) High-Risk Human Papillomavirus E7 Alters Host DNA Methylome and Represses HLA-E Expression in Human Keratinocytes. Sci Rep. 2017 Jun 16; 7(1):3633. PM ID: 28623356
  • Zhu, YX, et al. (2017) Loss of FAM46C promotes cell survival in myeloma. Cancer Res.. 2017 Jun 15;. PM ID: 28619709
  • Lin, X, et al. (2017) The Latency-Associated Nuclear Antigen of Kaposi’s Sarcoma-Associated Herpesvirus Inhibits Expression of SUMO/sentrin Specific Peptidase 6 to Facilitate Latency Establishment. J. Virol.. 2017 Jun 14;. PM ID: 28615201
  • Xia, M, et al. (2017) Ash1l and lnc-Smad3 coordinate Smad3 locus accessibility to modulate iTreg polarization and T cell autoimmunity. Nat Commun. 2017 Jun 9; 8:15818. PM ID: 28598443
  • Hwangbo, C, et al. (2017) Modulation of Endothelial Bone Morphogenetic Protein Receptor Type 2 Activity by Vascular Endothelial Growth Factor Receptor 3 in Pulmonary Arterial Hypertension. Circulation. 2017 Jun 6; 135(23):2288-2298. PM ID: 28356442
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