pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector

Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Lentivector

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pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter, Cloning and Expression Lentivector

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pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter, Cloning and Expression Lentivector

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10 µg
CD811A-1
$ 522
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Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a GFP reporter.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:

  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors

pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector

With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:

  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter

Not sure which promoter is right for you? This table should help:

Promoter Expression Level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells

References

  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.


Citations

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  • Li, X, et al. (2017) MicroRNA‑146a‑5p enhances cisplatin‑induced apoptosis in ovarian cancer cells by targeting multiple anti‑apoptotic genes. Int. J. Oncol.. 2017 Jul 1; 51(1):327-335. PM ID: 28560455
  • Zhou, F, et al. (2017) AML1-ETO requires enhanced C/D box snoRNA/RNP formation to induce self-renewal and leukaemia. Nat. Cell Biol.. 2017 Jul 1; 19(7):844-855. PM ID: 28650479
  • Li, SH, et al. (2017) Osthole Stimulated Neural Stem Cells Differentiation into Neurons in an Alzheimer’s Disease Cell Model via Upregulation of MicroRNA-9 and Rescued the Functional Impairment of Hippocampal Neurons in APP/PS1 Transgenic Mice. Front Neurosci. 2017 Jun 29; 11:340. PM ID: 28659755
  • Biering, SB, et al. (2017) Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Cell Host Microbe. 2017 Jun 28;. PM ID: 28669671
  • Tang, H, et al. (2017) KLF4 is a tumor suppressor in anaplastic meningioma stem-like cells and human meningiomas. J Mol Cell Biol. 2017 Jun 26;. PM ID: 28651379
  • Gu, Z, et al. (2017) Increased PTPRA expression leads to poor prognosis through c-Src activation and G1 phase progression in squamous cell lung cancer. Int. J. Oncol.. 2017 Jun 23;. PM ID: 28656243
  • Franzetti, GA, et al. (2017) Cell-to-cell heterogeneity of EWSR1-FLI1 activity determines proliferation/migration choices in Ewing sarcoma cells. Oncogene. 2017 Jun 22; 36(25):3505-3514. PM ID: 28135250
  • Katayama, M, et al. (2017) Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J. Reprod. Dev.. 2017 Jun 21; 63(3):311-318. PM ID: 28331164
  • Guo, Y, et al. (2017) Gelsolin regulates proliferation, apoptosis and invasion in NK/T-cell lymphoma. biorxiv.org. 2017 Jun 20;. Link: biorxiv.org
  • Al-Khalaf, HH, et al. (2017) Obesity and p16(INK4A) down-regulation activate breast adipocytes and promote their pro-tumorigenicity. Mol. Cell. Biol.. 2017 Jun 19;. PM ID: 28630279
  • Ye, L, et al. (2017) miR-1290 Is a Biomarker in DNA-Mismatch-Repair-Deficient Colon Cancer and Promotes Resistance to 5-Fluorouracil by Directly Targeting hMSH2. Mol Ther Nucleic Acids. 2017 Jun 16; 7:453-464. PM ID: 28624221
  • Cicchini, L, et al. (2017) High-Risk Human Papillomavirus E7 Alters Host DNA Methylome and Represses HLA-E Expression in Human Keratinocytes. Sci Rep. 2017 Jun 16; 7(1):3633. PM ID: 28623356
  • Zhu, YX, et al. (2017) Loss of FAM46C promotes cell survival in myeloma. Cancer Res.. 2017 Jun 15;. PM ID: 28619709
  • Lin, X, et al. (2017) The Latency-Associated Nuclear Antigen of Kaposi’s Sarcoma-Associated Herpesvirus Inhibits Expression of SUMO/sentrin Specific Peptidase 6 to Facilitate Latency Establishment. J. Virol.. 2017 Jun 14;. PM ID: 28615201
  • Xia, M, et al. (2017) Ash1l and lnc-Smad3 coordinate Smad3 locus accessibility to modulate iTreg polarization and T cell autoimmunity. Nat Commun. 2017 Jun 9; 8:15818. PM ID: 28598443
  • Gianni, M, et al. (2017) RARα2 and PML-RAR similarities in the control of basal and retinoic acid induced myeloid maturation of acute myeloid leukemia cells.. Oncotarget. 2017 Jun 6; 8(23):37041-37060. PM ID: 27419624
  • Fu, L, et al. (2017) RNA editing of SLC22A3 drives early tumor invasion and metastasis in familial esophageal cancer. Proc. Natl. Acad. Sci. U.S.A.. 2017 Jun 6; 114(23):E4631-E4640. PM ID: 28533408
  • Hwangbo, C, et al. (2017) Modulation of Endothelial Bone Morphogenetic Protein Receptor Type 2 Activity by Vascular Endothelial Growth Factor Receptor 3 in Pulmonary Arterial Hypertension. Circulation. 2017 Jun 6; 135(23):2288-2298. PM ID: 28356442