Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and co-expressed GFP and puromycin resistance via a T2A element.
This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
|CMV||High||Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)|
|MSCV||High||Hematopoietic and stem cells|
|EF1α||Medium||Most cell types including primary cells and stem cells|
|PGK||Medium||Most cell types including primary cells and stem cells|
|UbC||Low||Most cell types including primary cells and stem cells|
- Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.