Placing the power of iPSCs into your hands
When you’re using stem cells for modeling disease, discovering and developing new drugs, or building novel cell-based therapeutics, reliable generation of iPSCs is critical. Even better if the iPSCs are transgene-free. With SBI’s Episomal iPSC Reprogramming System you can quickly and efficiently reprogram human or mouse cells for a range of in vivo applications.
- Ideal for in vivo studies—iPSC generation that’s virus-free and footprint-free
- Efficient—get 70% more iPSC colonies than when using standard retroviral methods
- Fast—iPSC colony formation is complete in 25 days
- Flexible—works with both feeder and feeder-free conditions
- Easy—a simple, hands-free workflow for reliable reprogramming
SBI’s Episomal iPSC Reprogramming System is based on the Epstein-Barr Nuclear Antigen-1 plasmid system (oriP/EBNA-1) that has been proven to reliably and efficiently generate iPSCs free from the risk of integrated transgenic sequences1. The system works efficiently with patient fibroblasts and blood cells2, and can even generate iPSCs from difficult-to-reprogram source cells like older patient fibroblasts (Figure 1).
The system contains Oct4, Sox2, Klf4, L-myc, Lin28, shRNA-p53, and miR302/367 cluster reprogramming factors, along with a GFP marker for monitoring transfection efficiency and plasmid loss over time. Unlike traditional plasmid systems, the oriP/EBNA-1 system replicates in synchrony with the host genome by anchoring itself to chromatin and replicating during each cell cycle divisions. The episomal plasmids are naturally lost at up to 5% per cell division cycle, with most cells losing the episome completely by passage fifteen.
- Okita K, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May; 8(5):409-12. PMID: 21460823.
- Schlaeger, et al. A comparison of non-integrating reprogramming methods. Nat Biotechnol. 2015 Jan; 33(1):58-63. PMCID: PMC4329913.