CAGs-mIL-23 Enhanced Episomal Vector (EEV)

Facilitate your studies of inflammation in mouse models with this EEV plasmid that provides constitutive, non-viral, sustained expression of the mouse IL-23 gene

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Mouse IL-23 Enhanced Episomal Expression Vector

100 µg
EEV651A-1
$ 745
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Overview

An easy-to-produce non-integrating option for constitutive mouse IL-23 expression

With the CAGs-mIL-23 Enhanced Episomal Vector (EEV), you can get sustained, long-lasting, and non-viral expression of the mouse IL-23 gene from a non-integrating vector. Driven by the constitutive CAG promoter, the CAGs-mIL-23 EEV works in most cell types, including primary cells and stem cells, and can even be directly injected into mice.

CAGs-mIL-23 Enhanced Episomal Vector (EEV)

With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. SBI’s EEV System, which is an enhanced version of the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) system, offers:

  • High levels of expression
  • Easy to clone formats
  • No special plasmid production
  • Nonviral, non-integrating technology
  • Constitutive and inducible vector formats

Supporting Data

Sustained, non-integrating expression of mouse IL-23 in vivo via EEV

A constitutive EEV reporter based on CAGs-MCS leads to higher serum IL-23 levels in mice than two other episomal vector systems

Figure 1. A constitutive EEV reporter based on CAGs-MCS leads to higher serum IL-23 levels in mice than two other episomal vector systems. We compared SBI’s EEV system to two other episomal vector systems—minicircle technology1 and the mini-intronic plasmid system2—using a mouse IL-23 cDNA cloned into the three different vectors. We introduced the three vectors or vehicle only into mice using hydrodynamic tail vein delivery (HDD). After seven days, we measured serum IL-23 levels using an ELISA assay and found that the EEV technology outperformed the other episomal platforms by at least 10-fold, demonstrating the ability of the EEV platform to provide sustained, long-lasting transgene expression.

References

  1. Kay MA, He CY, and Chen ZY. A robust system for production of minicircle DNA vectors. Nat Biotechnol. 2010 Dec; 28(12):1287-9. PMCID: PMC4144359.
  2. Lu J, Zhang F, and Kay MA. A mini-intronic plasmid (MIP): a novel robust transgene expression vector in vivo and in vitro. Mol Ther. 2013 May; 21(5):954-63. PMCID: PMC3666631.