CAGs-GFP-T2A-Luciferase Enhanced Episomal Vector (EEV)

Get sustained, non-viral constitutive expression of GFP and luciferase reporters with this EEV plasmid and avoid the challenges of viral transduction

Description
Size
Catalog Number
Price
Quantity
Add to Cart

CAGs-GFP-T2A-Luciferase Dual Reporter Enhanced Episomal Vector (EEV) (Replaces EEV604A-1)

Array ( [attributes] => Array ( [attribute_size] => 10 µg ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 604 [display_regular_price] => 604 [image] => Array ( [title] => cags-gfp-t2a-luc-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01.png [full_src_w] => 2501 [full_src_h] => 2501 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/cags-gfp-t2a-luc-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 11112 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $604 [sku] => EEV604A-2 [variation_description] =>

CAGs-GFP-T2A-Luciferase Dual Reporter Enhanced Episomal Vector (EEV) (Replaces EEV604A-1)

[variation_id] => 63636 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
EEV604A-2
$ 604
Contact Us Speak to a specialist
1-888-266-5066

Overview

An easy-to-produce non-integrating option for constitutive GFP and luciferase expression

With virtually no limits on insert size (unlike AAV vectors) Enhanced Episomal Vectors (EEVs) are an excellent choice for non-integrating, non-viral gene expression. Because they replicate in synchrony with the host cell, they are stably inherited and can be used for long-lasting expression—up to several months both in vitro and in vivo—without modifying the host genome. Use SBI’s CAGs-GFP-T2A-Luciferase EEV Vector (Cat.# EEV604A-2) for constitutive, CAG-driven coordinated expression of GFP and luciferase reporters in most cell types, including primary cells and stem cells.

CAGs-GFP-T2A-Luciferase Enhanced Episomal Vector (EEV)

SBI’s EEV System, which is an enhanced version of the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA1) system, offers:

  • High levels of expression
  • Easy to clone formats
  • No special plasmid production
  • Nonviral, non-integrating technology
  • Constitutive and inducible vector formats

Supporting Data

Sustained, non-integrating gene expression in vitro and in vivo with EEV

A constitutive EEV reporter based on CAGs-MCS delivers similar amounts of GFP expression after 2-days and 11-days

Figure 1. A constitutive EEV reporter based on CAGs-MCS delivers similar amounts of GFP expression in vitro after two days and eleven days. SBI’s EEV reporter plasmid CAGs-GFP-Luciferase (Cat.# EEV604A-2), contains a GFP-T2A-Luciferase cassette cloned into the CAGs-MCS EEV Vector (Cat.# EEV601A-1). This plasmid was transfected into HEK293T cells (0.5 µg of CAGs-GFP-Luciferase in a 24-well plate) and GFP transgene expression imaged. There is little change in GFP signal between two- and eleven-days post-transfection.

A constitutive EEV reporter based on CAGs-MCS delivers high levels of expression in mice over forty days

Figure 2. A constitutive EEV reporter based on CAGs-MCS delivers high levels of expression in mice over forty days. 8 µg of the constitutive CAGs-GFP-T2A-Luc construct (Cat.# EEV604A-2) was introduced into test mice through hydrodynamic tail vein injection (HDD), a procedure which leads to high plasmid DNA transfection of the livers of mice in vivo. The test mice (n=3) were imaged for body luminescence in the liver area post-HDD from Day 1 up until Day 80. The results show that robust EEV-expressed luciferase expression is readily detectable at very high levels through day 40.