Get started with the SparQTM Cumate Switch System
We’ve made it easy to get started with the SparQTM Cumate Switch System, with the SparQ Cumate Switch Starter Pack. The Starter Pack includes all three components of the SparQ Cumate Switch System so you can quickly get cumate-inducible expression of your gene- or miRNA-of-interest into your lab.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Versatile—choose from all-in-one formats that co-express CymR and your gene-of-interest, or two-vector systems where CymR is expressed from a different plasmid
- Powerful—suitable for in vivo applications
With SBI’s SparQTM Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System is an excellent choice for achieving controlled levels of gene expression.
What’s included in the SparQ Cumate Switch Starter Pack?
The Starter Pack includes:
- CymR expression lentivector pCDH-EF1α-CymR-T2A-Puro as pre-packaged virus (# QM200VA-2)
- Cumate Solution (1,000x; # QM100A-1)
- And any two-component SparQ Cloning and Expression Lentivector (the All-in-one SparQ Lentivectors Cat.# QM800A-1 and QM812B-1 are not eligible to be included in the SparQ Cumate Switch Starter Pack)
- pCDH-CuO-MCS (# QM500A-1)
- pCDH-CuO-MCS-EF1α-GFP (# QM511B-1)
- pCDH-CuO-MCS-EF1α-RFP (# QM512B-1)
- pCDH-CuO-MCS-EF1α-GFP+Puro (# QM513B-1)
- pCDH-CuO-MCS-EF1α-RFP+Puro (# QM516B-2)
- pCDH-CuO-MCS-T2A-GFP (# QM521A-1)
- pCDH-CuO-MCS-T2A-RFP (# QM522A-1)
- pCDH-CuO-MCS-IRES-GFP (# QM530A-2)
- pCDH-CuO-MCS-IRES-RFP (# QM531A-2)