96-well ExoELISA Plate

Pack of ten 96-well ExoELISA plates optimized for use with any of SBI’s ExoELISA assays.

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96-well ExoELISA plate (12×8-well strips, pack of 10 plates)

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96-well ExoELISA plate (12×8-well strips, pack of 10 plates)

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10 Plates
EXOEL-96P-1
$ 143
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Overview

Additional plates optimized for our ExoELISA Kits

For researchers who just need additional ExoELISA plates, we offer 96-well plates as a pack of ten. Each 96-well plate comes as a 12 strips of 8 wells each to maximize your flexibility—unused strips can be stored at room temperature for later use.

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet

How It Works

Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.

The ExoELISA assay uses a colorimetric, HRP activity-based readout using extra-sensitive TMB as the substrate.

The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody (anti-CD9, -CD63, or -CD81). Following addition of the primary antibody, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of the exosome marker (CD9, CD63, or CD81) is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of marker present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying the marker of interest from the ExoELISA data.An example ExoELISA CD9 calibration curve using the included exosome standards.An example ExoELISA CD63 calibration curve using the included exosome standards.An example ExoELISA CD81 calibration curve using the included exosome standards.The number of exosome particles in the standards supplied with each ExoELISA Kit are quantified via NanoSight Analysis.

Citations

  • Elshelmani, H & Rani, S. (2017) Exosomal MicroRNA Discovery in Age-Related Macular Degeneration. Methods Mol. Biol.. 2017 Nov 9; 1509:93-113. PM ID: 27826921
  • Patel, DB, Gray, KM & Santharam, Y. (2017) Impact of Cell Culture Parameters on Production and Vascularization Bioactivity of Mesenchymal Stem Cell‐Derived Extracellular Vesicles. Bioengineering & Translational Medicine. 2017 Jun 26;. Link: Bioengineering & Translational Medicine
  • Li, Y, et al. (2017) Comparative Gene Expression Analysis of Lymphocytes Treated with Exosomes Derived from Ovarian Cancer and Ovarian Cysts. Front Immunol. 2017 Jun 16; 8:607. PM ID: 28620375
  • Xia, Y, et al. (2017) A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes. Biosens Bioelectron. 2017 Jun 15; 92:8-15. PM ID: 28167415
  • Nocera, AL, et al. (2017) Exosomes mediate interepithelial transfer of functional P-glycoprotein in chronic rhinosinusitis with nasal polyps. Laryngoscope. 2017 May 9;. PM ID: 28485529
  • Desgagné, V, et al. (2017) Changes in high-density lipoprotein-carried miRNA contribution to the plasmatic pool after consumption of dietary trans fat in healthy men. Epigenomics. 2017 May 1; 9(5):669-688. PM ID: 28470118
  • Tan, DB, et al. (2017) Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation. Respiratory Medicine. 2017 Apr 26;. Link: Respiratory Medicine
  • Mead, B & Tomarev, S. (2017) Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms. Stem Cells Transl Med. 2017 Apr 1; 6(4):1273-1285. PM ID: 28198592
  • Barclay, RA, et al. (2017) Isolation of Exosomes from HTLV-Infected Cells. Methods Mol. Biol.. 2017 Mar 30; 1582:57-75. PM ID: 28357662
  • Tao, SC, et al. (2017) Chitosan Wound Dressings Incorporating Exosomes Derived from MicroRNA-126-Overexpressing Synovium Mesenchymal Stem Cells Provide Sustained Release of Exosomes and Heal Full-Thickness Skin Defects in a Diabetic Rat Model. Stem Cells Transl Med. 2017 Mar 1; 6(3):736-747. PM ID: 28297576
  • Hurwitz, SN, et al. (2017) CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-κB Signaling. J. Virol.. 2017 Mar 1; 91(5). PM ID: 27974566
  • Woo, HK, et al. (2017) Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from Biological Samples. ACS Nano. 2017 Feb 28; 11(2):1360-1370. PM ID: 28068467
  • Galazka, G, et al. (2017) Multiple sclerosis: Serum-derived exosomes express myelin proteins. Mult. Scler.. 2017 Feb 1;:1352458517696597. PM ID: 28273783
  • Bidarimath, M, et al. (2017) Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk. Sci Rep. 2017 Jan 12; 7:40476. PM ID: 28079186
  • Guo, SC, et al. (2017) Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model. Theranostics. 2017 Jan 2; 7(1):81-96. PM ID: 28042318
  • Tao, SC, et al. (2017) Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model. Theranostics. 2017 Jan 2; 7(1):180-195. PM ID: 28042326
  • Erkan, EP, et al. (2017) Extracellular vesicle-mediated suicide mRNA/protein delivery inhibits glioblastoma tumor growth in vivo. Cancer Gene Ther.. 2017 Jan 1; 24(1):38-44. PM ID: 27982017
  • Hosseini, M, et al. (2016) Exosome-encapsulated microRNAs as potential circulating biomarkers in colon cancer.. Curr. Pharm. Des.. 2016 Dec 1;. PM ID: 27908272
  • Xu, R, et al. (2016) Quantification of Small Extracellular Vesicles by Size Exclusion Chromatography with Fluorescence Detection. Anal. Chem.. 2016 Nov 1; 88(21):10390-10394. PM ID: 27689436
  • Zhang, J, et al. (2016) Exosomal Long Noncoding RNAs are Differentially Expressed in the Cervicovaginal Lavage Samples of Cervical Cancer Patients. J. Clin. Lab. Anal.. 2016 Nov 1; 30(6):1116-1121. PM ID: 27184657