Exosome Cyto-Tracer, pCT-CD63-RFP

Label exosomes with RFP by expressing the pCT-CD63-RFP Cyto-Tracer in the parent cells. Great for tracking exosomes and monitoring cargo delivery.

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pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)

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pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag)

[variation_id] => 63611 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
CYTO120R-PA-1
$ 558

pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag; pre-packaged virus)

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pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag; pre-packaged virus)

[variation_id] => 63612 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2x10^6 IFUs
CYTO120R-VA-1
$ 653
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Overview

Easily track exosomes with a CD63-RFP fusion

Monitor exosome dynamics with the pCT-CD63-RFP (pCMV) Exosome Cyto-Tracer (also available as a CD63-GFP fusion, pCT-CD63-GFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses RFP to the tetraspanin CD63, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce RFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.

  • Stable lentivector-based system
  • Ideal for co-localization studies
  • Monitor exosome dynamics and functional studies in real time
  • Label exosomes from primary cells, tumor cells, stem cells, and more

Choose the exosome quantitation method that’s best for your studies

Promoter (Cat.#) Cyto-Tracer Target Location Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFP Plasma Membrane Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFP Inner Leaflet of Membrane Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFP Mitochondria Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1) pCT-ER-GFP Endoplasmic Reticulum ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1) pCT-Golgi-GFP Golgi Apparatus Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1) pCT-Secretory-GFP ER-Golgi  Vesicles Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1) pCT-Lyso-GFP Lysosome Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1) pCT-Endo-GFP Endosome RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1) pCT-Endo-RFP Endosome RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFP Peroxisome Peroxisomal consensus signal
CMV (CYTO120-PA-1/CYTO120-VA-1) pCT-CD63-GFP Autophagosome LC3
CMV (CYTO120R-PA-1/CYTO120R-VA-1) pCT-CD63-RFP Autophagosome LC3
CMV (CYTO109-PA-1/CYTO109-VA-1) pCT-Autophago-GFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO109R-PA-1/CYTO109R-VA-1) pCT-Autophago-RFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1) pCT-CD9-GFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1) pCT-CD9-RFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1) pCT-CD81-GFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1) pCT-CD81-RFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFP Cytoskeleton β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFP Cytoskeleton α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1) pCT-Tublin-RFP Cytoskeleton α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1) pCT-MAP4-GFP Microtubule Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFP Nucleus H2B
CMV (CYTO114-PA-1/CYTO114-VA-1) pCT-Apoptosis-Luc  Caspase 3/7 activation Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1) pCT-GFP-BAX Cytosol to Mitochondria BAX
CMV (CYTO116-PA-1/CYTO116-VA-1) pCT- Catenin-GFP Cytosol to Nucleus/Cell Membrane β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1) pCT-CMV-PSD95-GFP Dendrite Membranes PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFP Cytosolic Compartment GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1) pCT-Cyto-RFP Cytosolic Compartment RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1) pCT-Cyto-GFpLuc GFP and Luciferase Control GFP and Luciferase (Untagged)

Supporting Data

See some of our exosome Cyto-Tracers in action

The following figure and videos are from:

Garcia NA, et al. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells. PLoS ONE. 2015. 10(9). PMCID: PMC4578916.

See exosomes being transferred from cardiomyocytes to mouse endothelial cells and human cord blood endothelial cells (HUVEC)

Figure 4 from Garcia, et al. Exosome transfer from CMs to ECs. (A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.

Video S1 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.

Video S2 from Garcia, et al. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.

Exosomes labeled with CD63-GFP (top panels) and co-transfected with CD9-GFP and CD9-RFP (bottom panels)

Labeled exosomes. (Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-tracers co-transfected into HEK293 cells.


Citations

  • Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
  • Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
  • Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
  • Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
  • Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015 Jun 24; 6:7506. PM ID: 26103923
  • Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014 Nov 26; 5:5513. PM ID: 25423885
  • Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
  • Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014 Nov 4; 5:5266. PM ID: 25366663
  • Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. ;. Link: Thesis
  • Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. ;. Link: Thesis
  • VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. ;. Link: Thesis
  • Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. ;. Link: Thesis