ExoQuick-TC

Fast, scalable exosome isolation from tissue culture media

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ExoQuick-TC

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ExoQuick-TC

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10 mL
EXOTC10A-1
$ 307

ExoQuick-TC

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ExoQuick-TC

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50 mL
EXOTC50A-1
$ 1031
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Overview

A better way to isolate exosomes

“We therefore pursued the ExoQuick® method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models1.”
 
Need exosomes? SBI’s ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). Note, to isolate exosomes from serum, plasma, or ascites fluid, use the original ExoQuick formulation (EXOQ5A-1 or EXOQ20A-1)). Compatible with a wide variety of downstream applications, ExoQuick-TC is an effective and proven alternative to ultracentrifugation1-3.

ExoQuick-TC’s fast, ultracentrifugation-free method:

  • Saves time and labor
  • Is easily scalable
  • Conserves precious sample
  • Delivers high yields of functional, high quality exosomes
  • Can be used to isolate exosomes for a wide range of downstream applications, including
    • Biomarker studies
    • Exosomal miRNA profiling
    • Exosomal proteomics
    • Exosomal lipidomics/metabolomics
    • Functional studies, such as in cell-to-cell signaling
    • Basic biology, such as role in tumorigenesis

ExoQuick-TC is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick-TC to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.

Biofluid Sample volume ExoQuick-TC Volume
Tissue culture media, urine, cerebrospinal fluid (CSF), etc. 5 mL 1 mL

In electron microscopy studies, exosomes isolated with ExoQuick-TC appear similar to exosomes isolated using ultracentrifugation1-2, and these exosomes are also active in numerous functional assays1-3.

Exosomes isolated with ExoQuick-TC can be used for all types of protein profiling and protein characterization studies, such as mass spectrometry, Western blotting, ELISA, and more. Higher protein yields are achieved by ExoQuick purification than by chromatography, DynaBeads, or ultracentrifugation.

Exosomes isolated with ExoQuick-TC also provide excellent samples for studying exosome-associated nucleic acids such as microRNAs, siRNAs, and even mRNA. Quantitative analytical techniques such as qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-TC-purified exosomes.

Backed by a growing number of publications, ExoQuick-TC is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models.

ExoQuick-TC exosome isolation methods are patented technologies4.

Choose the right ExoQuick for your application:

Application Product Catalog #
Purest EV isolation ExoQuick ULTRA and
ExoQuick-TC ULTRA
EQULTRA-20A-1
EQULTRA-20TC-1
General purpose EV isolation ExoQuick and
ExoQuick-TC
EXOQ20A-1
EXOTC50A-1
EV isolation for pre-clinical/in vivo studies ExoQuick-CG EXOCG50A-1
EV isolation that removes contaminating lipoprotein particles from plasma or serum ExoQuick-LP EXOLP5A-1
EV isolation that includes a de-fibrinating plasma step prior to isolation ExoQuick Plasma Prep with Thrombin EXOQ5TM-1

REFERENCES

  1. Chugh PE, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog. 2013. 9(7): e1003484. PMCID: PMC3715412.
  1. Umezu T, et al. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2013 May 30. 32(22):2747-55. PMID: 22797057.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Antes T, et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.

How It Works

High-throughput, quantitative exosome recovery

ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media4, and from certain biofluids such as saliva1, urine2, follicular fluid3, and breast milk5. With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick-TC is an excellent option for researchers who need to purify multiple exosome samples.

To isolate exosomes from tissue culture media, simply:

  • Add an appropriate volume of ExoQuick-TC
  • Incubate overnight at 4°C
  • Isolate exosomes with a 30-minute low-speed spin (1500g).

Isolated exosomes can be found in the pellet and resuspended in an appropriate solution.

A quick and easy exosome isolation workflow

You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn about different ways to detect exosomes and more in our Exosome Basics Guide).

The Bottom Line
With ExoQuick-TC, you can obtain high-quality exosomes from tissue culture media and certain biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.

REFERENCES

  1. Yang J, et al. Detection of Tumor Cell-Specific mRNA and Protein in Exosome-Like Microvesicles from Blood and Saliva. PLoS ONE. 2014. 9(11): e110641. PMCID: PMC 4232306.
  1. Alvarez ML. Isolation of urinary exosomes for RNA biomarker discovery using a simple, fast, and highly scalable method. Methods Mol Biol. 2014. 1182:145-70. PMID: 25055908.
  1. Sohel MM, et al. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4. 8(11):e78505. PMCID: PMC3817212.
  1. Zhu L, et al. Novel method for extracting exosomes of hepatocellular carcinoma cells. World J Gastroenterol. 2014 June 7. 20(21): 6651-6657. PMCID: PMC4047354.
  1. Gu Y, et al. Lactation-Related MicroRNA Expression Profiles of Porcine Breast Milk Exosomes. PLoS ONE. 2012. 7(8): e43691. PMCID: PMC3427246.

Supporting Data

Characterizing ExoQuick-TC exosomes with NanoSight

Exosomes purified with ExoQuick-TC from tissue culture media show the expected particle size distribution and high concentration yields when analyzed using NanoSight’s Nanoparticle Tracking Analysis (NTA, Figure 1).

ExoQuick-TC delivers high yields of particles consistent in size with exosomes

Figure 3.Exosome size distribution and yields from tissue culture media. Cells from a human HT1080 lung sarcoma cell line were cultured in conditioned media (serum-free) for 72 hours. 10 mL of media was combined with 2 mL ExoQuick-TC, incubated overnight at 4°C, and centrifuged at 1500g for 30 minutes to isolate exosomes. The exosome pellet was resuspended in 1 mL PBS, diluted 1:40, and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick-TC recovered 133 nm exosomes at a concentration of 1.74 x 109 particles/mL.


Product Documentation

Citations

  • McNicholas, K & Michael, MZ. (2017) Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis. Methods Mol. Biol.. 2017 Dec 12; 1545:35-42. PM ID: 27943205
  • Slowey, PD. (2017) Salivary Diagnostics Using Purified Nucleic Acids. Methods Mol. Biol.. 2017 Dec 7; 1537:3-15. PM ID: 27924585
  • Belo, R, et al. (2017) Leishmania infantum Exoproducts Inhibit Human Invariant NKT Cell Expansion and Activation. Front Immunol. 2017 Jul 4; 8:710. PM ID: 28674535
  • Hosseinkhani, B, et al. (2017) Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance. Nanomedicine. 2017 Jul 1; 13(5):1663-1671. PM ID: 28366819
  • Tritten, L, et al. (2017) Excretory/secretory products from the gastrointestinal nematode Trichuris muris. Exp. Parasitol.. 2017 Jul 1; 178:30-36. PM ID: 28533110
  • Willis, CM, et al. (2017) A Refined Bead-Free Method to Identify Astrocytic Exosomes in Primary Glial Cultures and Blood Plasma. Front Neurosci. 2017 Jun 30; 11:335. PM ID: 28663721
  • Cheng, L, et al. (2017) Exosomes from Melatonin Treated Hepatocellularcarcinoma Cells Alter the Immunosupression Status through STAT3 Pathway in Macrophages. Int. J. Biol. Sci.. 2017 Jun 28; 13(6):723-734. PM ID: 28655998
  • Sáenz-Cuesta, M, et al. (2017) Proteomic Analysis of Extracellular Vesicles in Neurological Diseases. Current Proteomic Approaches Applied to Brain Function. 2017 Jun 28; 127:245-253. Link: Current Proteomic Approaches Applied to Brain Function
  • Kastelowitz, N, et al. (2017) Peptides derived from MARCKS block coagulation complex assembly on phosphatidylserine. Sci Rep. 2017 Jun 27; 7(1):4275. PM ID: 28655899
  • Yang, Y, et al. (2017) Exosomes from Plasmodium-infected hosts inhibit tumor angiogenesis in a murine Lewis lung cancer model. Oncogenesis. 2017 Jun 26; 6(6):e351. PM ID: 28650446
  • Nakata, R, et al. (2017) Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells. Journal of Extracellular Vesicles. 2017 Jun 25; 6(1). Link: Journal of Extracellular Vesicles
  • Golan-Gerstl, R, et al. (2017) Characterization and biological function of milk-derived miRNAs. Mol Nutr Food Res. 2017 Jun 23;. PM ID: 28643865
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Osawa, S, et al. (2017) Fibronectin on extracellular vesicles from microvascular endothelial cells is involved in the vesicle uptake into oligodendrocyte precursor cells. Biochem. Biophys. Res. Commun.. 2017 Jun 17; 488(1):232-238. PM ID: 28499870
  • Lee, YS, et al. (2017) Exosomes derived from palmitic acid-treated hepatocytes induce fibrotic activation of hepatic stellate cells. Sci Rep. 2017 Jun 16; 7(1):3710. PM ID: 28623272
  • Zhao, L, et al. (2017) Isolation and Identification of miRNAs in exosomes derived from serum of colon cancer patients. J Cancer. 2017 Jun 13; 8(7):1145-1152. PM ID: 28607588
  • Markowska, A, et al. (2017) A novel method for the isolation of extracellular vesicles and RNA from urine. Journal of Circulating Biomarkers. 2017 Jun 13;. Link: Journal of Circulating Biomarkers
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Quan, Y, et al. (2017) Exosome miR-371b-5p promotes proliferation of lung alveolar progenitor type II cells by using PTEN to orchestrate the PI3K/Akt signaling. Stem Cell Res Ther. 2017 Jun 8; 8(1):138. PM ID: 28595637
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875