Quick and efficient exosome isolation for sensitive applications
While the ExoQuick® family of reagents speed-up and simplify exosome isolation and are compatible with most downstream uses, some applications are especially sensitive to carry-over of non-exosomal proteins—applications such as mass spectrometry, Western blotting of low abundance markers, exosome labeling, and in vivo /ex vivo exosome delivery. Which is why SBI offers ExoQuick PLUS.
Less is more—ExoQuick PLUS and ExoQuick-TC PLUS for when you need an extra level of confidence:
- Better—delivers as much as 150-fold higher yields than ultracentrifugation-based methods
- Cleaner—dramatically reduces the amount of carry-over protein compared to other column- or precipitation-based kits, and even ultracentrifugation-based methods
- Powerful—detects biomarkers with as much as a 3-fold increase in sensitivity
- Fast—takes less than 10 minutes of hands-on-time after initial ExoQuick isolation
Building on our popular ExoQuick family of products, ExoQuick PLUS delivers dramatically reduced albumin contamination in serum/plasma preparations compared to utracentrifugation-based isolation methods (Supporting Data, Figure 1), and considerably less carryover protein compared to other exosome isolation kits (Supporting Data, Figure 2). Even better, isolation with the ExoQuick PLUS family of products results in much higher yields than isolation with ultracentrifugation—as much as 150-fold higher (Supporting Data, Figure 3).
With a simple treatment of our ready-to-use, customized ExoQuick PLUS Microspheres (kits contain enough ExoQuick and pre-packed Microspheres for 10 reactions*), you can obtain highly pure, intact exosomes suitable for a variety of protein-sensitive applications. Total hands-on time after ExoQuick isolation is less than 10 minutes!
*For exosome isolation from serum or plasma, one reaction is defined as 250 µL of serum or plasma. For exosome isolation from tissue culture media, one reaction is defined as 5 mL of media.
Learn more about ExoQuick—click here.
Choose the right ExoQuick for your biofluid:
|EXOQ5A-1, EXOQ20A-1||The Original ExoQuick||For serum, plasma, and ascites fluid|
|EXOTC10A-1, EXOTC50A-1||ExoQuick-TC®||For all other biofluids|
|EXOLP5A-1||ExoQuick-LP||For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation|
|EXOQ5TM-1||ExoQuick Plasma Prep with Thrombin||For de-fibrinating plasma before exosome isolation for efficient recovery and high yields|
|EXOCG50A-1||ExoQuick-CG||For preparing exosomes used in pre-clinical in vivo applications|
|EQPL10A-1, EQPL10TC||ExoQuick PLUS and ExoQuick-TC PLUS||For sensitive applications such as mass spectrometry, exosome labeling, and in vivo/ex vivo exosome delivery|
Choose between ExoQuick/ExoQuick-TC and ExoQuick PLUS/ExoQuick-TC PLUS based on your application
|Western blotting for general exosome markers (e.g. CD9, CD63, CD81, TSG101, Alix)||•••••||•••••|
|High-sensitivity Western blotting (e.g. low abundance biomarkers)||•••||•••••|
|qPCR of coding and non-coding RNAs (e.g. mRNA, miRNA, and lncRNA)||•••••||•••••|
High-throughput Biomarker Discovery
|RNA-seq of exosomal RNAs||•••••||•••••|
|Mass spectrometry of exosomal proteins||•••||•••••|
|Lipidomics/metabolomics of exosomal cargo||•••||•••••|
|In vivo/ex vivo Exosome Delivery||•••||•••••|
••••• Highly recommended, • Not recommended