Exo-FlowMag96 96-well Magnetic Plate

96-well magnetic plate for Exo-Flow IP Kits—high-throughput, plate-based immunopurification of exosomes from serum, plasma, media, and other biofluids

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Exo-FlowMag96 96-well magnetic plate

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Exo-FlowMag96 96-well magnetic plate

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1 Kit
EXOFLOWMAG-1
$ 571
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Overview

Simplify your bead-based immunopurifications

Validated to work with any of our Exo-Flow32 or Exo-Flow96 IP Kits, the Exo-FlowMag96 Magnetic Plate simplifies plate-based exosome isolation using magnetic beads.

Use the Exo-Flow Multifunctional Magnetic Stand with any of our Exo-Flow Capture Kits:

Cat.# Kit
EXOFLOW100A-1 CD9 Exo-Flow Capture Kit
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo Capture Kit

EXOFLOW200A-1 CD31 Exo-Flow Capture Kit
EXOFLOW210A-1 CD44 Exo-Flow Capture Kit
EXOFLOW300A-1 CD63 Exo-Flow Capture Kit
EXOFLOW400A-1 CD81 Exo-Flow Capture Kit
EXOFLOW500A-1 Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1 HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1 CD14 Exo-Flow Capture Kit
EXOFLOW620A-1 CD68 Exo-Flow Capture Kit
EXOFLOW630A-1 EpCAM Exo-Flow Capture Kit
EXOFLOW650A-1 α2-Macroglobulin (A2M) Exo-Flow Capture Kit
EXOFLOW660A-1 CD73 Exo-Flow Capture Kit

How It Works

Take a closer look at the Exo-FlowMag96 96-well Magnetic Plate

How the Exo-FlowMag96 96-well magnetic plate works


Once the Exo-Flow32 or Exo-Flow96 clear plastic plate is placed into the Exo-FlowMag96 96-well Magnetic Plate, the magnetic beads stick to the bottom of each well simplifying removal of buffer for washing and elution.

 

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particlesNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particlesNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.


Citations

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  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle. AIDS. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
  • Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies.. Eur. Urol.. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
  • Mukherjee, K, et al. (2016) Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response. EMBO Rep.. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
  • Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
  • Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases.. Expert Rev Proteomics. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
  • Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
  • Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition. Phys Biol. 2014 Nov 26; 11(6):065001. PM ID: 25426818
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  • Basu, S & Bhattacharyya, SN. (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells. Nucleic Acids Res.. 2014 Jun 1; 42(11):7170-85. PM ID: 24813441
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