Exo-Flow32 CD9 IP Kit

For high-throughput, 32-well plate-based CD9 immunopurification of exosomes from serum, plasma, media, or other biofluids

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Exo-Flow32 CD9 IP kit

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Exo-Flow32 CD9 IP kit

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32 Reactions
EXOFLOW32A-CD9
$ 527
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Overview

Streamline affinity-based exosome immunopurification

With magnetic beads already pre-coupled to anti-CD9 antibodies and delivered in a 32-well format, SBI’s Exo-Flow32 CD9 IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD9 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.

  • Selection of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation

Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.

Exosome capture is highly efficient with large bead size

Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).The optional Exo-FlowMag96 simplifies Exo-Flow IP Kit isolations

Different formats for different needs

To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.

Cat.# Kit
EXOFLOW32A-CD63 Exo-Flow32 CD63 IP kit
EXOFLOW32A-CD81 Exo-Flow32 CD81 IP kit
EXOFLOW32A-CD9 Exo-Flow32 CD9 IP kit
EXOFLOW32A-Tetra Exo-Flow32 Tetra IP kit (CD9, CD63, CD81)
EXOFLOW96A-CD63 Exo-Flow96 CD63 IP kit
EXOFLOW96A-CD81 Exo-Flow96 CD81 IP kit
EXOFLOW96A-CD9 Exo-Flow96 CD9 IP kit
EXOFLOW96A-Tetra Exo-Flow96 Tetra IP kit (CD9, CD63, CD81)

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.


Citations

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  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
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  • Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
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  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
  • Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
  • Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition. Phys Biol. 2014 Nov 26; 11(6):065001. PM ID: 25426818
  • Di Noto, G, et al. (2014) Immunoglobulin Free Light Chains and GAGs Mediate Multiple Myeloma Extracellular Vesicles Uptake and Secondary NfκB Nuclear Translocation. Front Immunol. 2014 Nov 11; 5:517. PM ID: 25386176
  • Basu, S & Bhattacharyya, SN. (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells. Nucleic Acids Res.. 2014 Jun 1; 42(11):7170-85. PM ID: 24813441
  • Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics. Expert Rev. Mol. Diagn.. 2014 Apr 1; 14(3):307-21. PM ID: 24575799
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  • Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes. Thesis. ;. Link: Thesis
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product