Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain
Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.
However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.
Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.
Use Exo-APC with any of our Exo-Flow Capture Kits:
|EXOFLOW100A-1||CD9 Exo-Flow Capture Kit|
|EXOFLOW150A-1||Tetraspanin Exo-Flow Combo Capture Kit|
|EXOFLOW200A-1||CD31 Exo-Flow Capture Kit|
|EXOFLOW210A-1||CD44 Exo-Flow Capture Kit|
|EXOFLOW300A-1||CD63 Exo-Flow Capture Kit|
|EXOFLOW400A-1||CD81 Exo-Flow Capture Kit|
|EXOFLOW500A-1||Rab5b Exo-Flow Capture Kit|
|EXOFLOW600A-1||HLA-G Exo-Flow Capture Kit|
|EXOFLOW610A-1||CD14 Exo-Flow Capture Kit|
|EXOFLOW620A-1||CD68 Exo-Flow Capture Kit|
|EXOFLOW630A-1||EpCAM Exo-Flow Capture Kit|
|EXOFLOW650A-1||α2-Macroglobulin (A2M) Exo-Flow Capture Kit|
|EXOFLOW660A-1||CD73 Exo-Flow Capture Kit|