Exo-APC Exosome FACS Stain

An APC-based stain to visualize exosomes immobilized to Exo-Flow beads for FACS and other imaging applications

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW810A-1 Exo-APC Exosome FACS stain 20 Reactions $195
- +

Overview

Overview

Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain

Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.

However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.

Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.

Use Exo-APC with any of our Exo-Flow Capture Kits:
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with our Exo-Flow Capture Kits

At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-APC (excitation and emission wavelengths of 650 nm and 660 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-APC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See Exo-APC in action

Highly selective exosome isolation by FACS using Exo-APC stain

Exo-APC enables selective exosome isolation via FACS. Exosomes are isolated using the CD9 Exo-Flow Capture Kit and flow-sorted using APC-positive gating.

FAQs

Resources

Citations

  • Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
  • Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
  • Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
  • Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
  • Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
  • Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
  • Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
  • Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
  • Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
  • Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
  • Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
  • Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
  • Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
  • Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
  • Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
  • Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
  • Beatriz, M, et al. (2022) Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington’s disease. Journal of Extracellular Biology. 2022; 1(10). Link: Journal of Extracellular Biology
  • Zhang, H, et al. (2021) Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials. Membranes. 2021; 12(1). PM ID: 35054584
  • Luo, ZW, et al. (2021) Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation. World journal of stem cells. 2021; 13(11):1762-1782. PM ID: 34909122
  • Zhang, Z, et al. (2021) Surface Located Adiponectin On Adipocytes-derived Exosomes Mediates Adipocytes/cardiomyocytes Communication And Contributes To Cardioprotection. Circulation. 2021;. Link: Circulation

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW810A-1 Exo-APC Exosome FACS stain 20 Reactions $195
- +

Overview

Overview

Visualize bead-immobilized exosomes with our proprietary, reversible APC-based stain

Optimized for our Exo-Flow Capture Kits and available only from SBI, Exo-APC is a proprietary reversible fluorescent stain for visualizing exosomes. Exo-APC is comprised of the commonly used fluorophore allophycocyanin (APC) conjugated to a protein that recognizes post-translational modifications on exosomal surface proteins, enabling general staining of exosomes.

However, the feature that really makes Exo-APC stand out as an ideal reagent for FACS-based isolation of exosomes is its reversibility—after staining with Exo-APC and flow-sorting by APC-positive gating (Exo-APC’s excitation and emission wavelengths are 650 nm and 660 nm, respectively), Exo-APC can be removed from exosomes through the simple addition of Exosome Elution Buffer, which simultaneously elutes exosomes from the Exo-Flow magnetic beads and removes bound Exo-APC.

Note that we also offer reversible Exo-FITC (Cat.# Exo-FLOW800A-1) as an alternative stain to Exo-APC.

Use Exo-APC with any of our Exo-Flow Capture Kits:
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with our Exo-Flow Capture Kits

At a glance
Simply (1) couple biotinylated antibody to the magnetic streptavidin beads, (2) use the antibody-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-APC (excitation and emission wavelengths of 650 nm and 660 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-APC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See Exo-APC in action

Highly selective exosome isolation by FACS using Exo-APC stain

Exo-APC enables selective exosome isolation via FACS. Exosomes are isolated using the CD9 Exo-Flow Capture Kit and flow-sorted using APC-positive gating.

FAQs

Citations

  • Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
  • Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
  • Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
  • Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
  • Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
  • Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
  • Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
  • Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
  • Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
  • Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
  • Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
  • Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
  • Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
  • Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
  • Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
  • Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
  • Beatriz, M, et al. (2022) Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington’s disease. Journal of Extracellular Biology. 2022; 1(10). Link: Journal of Extracellular Biology
  • Zhang, H, et al. (2021) Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials. Membranes. 2021; 12(1). PM ID: 35054584
  • Luo, ZW, et al. (2021) Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation. World journal of stem cells. 2021; 13(11):1762-1782. PM ID: 34909122
  • Zhang, Z, et al. (2021) Surface Located Adiponectin On Adipocytes-derived Exosomes Mediates Adipocytes/cardiomyocytes Communication And Contributes To Cardioprotection. Circulation. 2021;. Link: Circulation