XStampTM-NCAM Lentivector for Targeting Exosomes to neurons

Target exosomes to neurons with the pre-built XStamp-NCAM Lentivector Construct.

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XStamp NCAM brain targeting lentivector – uses NCAM to target exosomes to the brain

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XStamp NCAM brain targeting lentivector – uses NCAM to target exosomes to the brain

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10 µg
XSTP721PA-1
$ 683
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Overview

Putting exosomes to work: Target exosomes to neurons

Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to neurons with XStamp-NCAM. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-NCAM Lentivector fuses NCAM sequences to the XStamp tag, ensuring that exosomes display the NCAM homophilic binding domain on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-NCAM Lentivector will now be targeted to NCAM-expressing cells.

  • Display NCAM sequences on exosome surfaces
  • Target neurons
  • Create stable XStamp-NCAM cell lines

XStamp-NCAM places NCAM on the surface of your exosomes, targeting them to neurons

SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:

Cat.# Specificity Targeting Ligand Used
XSTP710PA-1 Clone-your-own You decide
XSTP720PA-1 GI tract cells Motilin
XSTP721PA-1 Neural cells NCAM
XSTP722PA-1 Brain cells BHP1
XSTP723PA-1 Cancer cells GE11
XSTP724PA-1 Breast cancer cells HER2
XSTP725PA-1 Antigen-presenting cells CD40L/CD154
XSTP726PA-1 Immune cells IL-2

How It Works

Engineering exosomes with specific surface proteins

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

See XStamp targeting in action

 
XStamp-NCAM and XStamp-BHP1 both target exosomes to neurons.

Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.