XStampTM-IL-2 Lentivector for Targeting Exosomes to Immune Cells

Target exosomes to immune cells with the pre-built XStamp-IL-2 Lentivector Construct.

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Xstamp IL-2 receptor targeting lentivector – IL-2 exosomes target cells with surface IL-2 receptor

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Xstamp IL-2 receptor targeting lentivector – IL-2 exosomes target cells with surface IL-2 receptor

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10 µg
XSTP726PA-1
$ 683
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Overview

Putting exosomes to work: Target exosomes to immune cells

Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to immune cells with XStamp-IL-2. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-IL-2 Lentivector fuses IL-2 sequences to the XStamp tag, ensuring that exosomes display IL-2 on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-IL-2 Lentivector will now be targeted to cells expressing the IL-2 receptor, primarily immune cells.

  • Display IL-2 sequences on exosome surfaces
  • Target immune cells
  • Create stable XStamp-IL-2 cell lines

XStamp-IL-2 places IL-2 sequences on the surface of your exosomes, targeting them to immune cells

SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:

Cat.# Specificity Targeting Ligand Used
XSTP710PA-1 Clone-your-own You decide
XSTP720PA-1 GI tract cells Motilin
XSTP721PA-1 Neural cells NCAM
XSTP722PA-1 Brain cells BHP1
XSTP723PA-1 Cancer cells GE11
XSTP724PA-1 Breast cancer cells HER2
XSTP725PA-1 Antigen-presenting cells CD40L/CD154
XSTP726PA-1 Immune cells IL-2

How It Works

Engineering exosomes with specific surface proteins

XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.

Supporting Data

See XStamp targeting in action

 
 
XStamp-motilin targets exosomes to GI cells.

Figure 1. XStamp with motilin specifically targets exosomes to GI cells. Motilin is a 22-amino acid polypeptide hormone that binds to the motilin receptor, which is exclusively expressed in the intestine. XStamp with motilin can be used to target exosomes to GI cells. METHODS: The XStamp-Motilin construct (Cat.# XSTP720PA-1) was transfected into HEK293 cells and after 48 hours, the exosomes were isoalted using ExoQuick-TC. The next day, the XStamp-Motilin exosomes were Exo-Fected with a Texas-Red-labeled siRNA to monitor exosome docking and delivery. The transfected XStamp-Motilin exosomes were then added to MDA-MB-231 Breast Cancer Cells (motilin receptor negative) and to HT-29 Colon Cancer Cells (motilin receptor positive). The cells were imaged after 24 hours for uptake of the Texas-Red-labeled siRNA delivery from the XStamped exosomes. The HT-29 colon cancer cells that are motilin receptor positive took up the XStamp-Motilin exosomes at a much higher rate than the MDA-MB-231 Breast Cancer (motilin receptor negative) cells.