Putting exosomes to work: Clone-your-own XMIRXpress Lentivector
Adding customization to our powerful XMIRXpress Lentivector System, SBI offers a linearized, ready-for-ligation version of the XMIRXpress Lentivector that does not contain coding sequence for an miRNA, so you can clone your own miRNA into the lentivector. Simply design two DNA oligos for the top and bottom strand, anneal the top and bottom oligos, and ligate directly into the XMIRXpress Cloning Lentivector.
Create stable cell lines that produce exosomes carrying your miRNA-of-interest and then use those exosomes to deliver that miRNA to target cells. Don’t forget that we also have a series of pre-built XMIRXpress Lentivectors for even easier miRNA-containing exosome production.
All XMIRXpress Lentivectors feature an EF1a-GFP-Puro selection cassette and a downstream H1 promoter expressing the XMIR + XMotif cassette.
- Flexible—turn the cell line of your choice into a factory that produces miRNA-packed exosomes
- Powerful—great for a range of applications, including:
- Modulating expression of miRNA-targets in recipient cells
- Developing exosome-delivered miRNA-based therapies