SeraMir Exosome RNA Amplification Kit for Media & Urine

Everything you need to accurately and sensitively measure exosomal RNAs from media, urine, and other biofluids (except serum, plasma, and ascites fluid).

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Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components)

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Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components)

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10 Reactions
RA800TC-1
$ 535
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Overview

Profile exosomal RNA biomarkers and more

Looking for biomarkers? SBI’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).

  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Excellent preparation method for qPCR
  • Can be used to amplify sense-strand exoRNAs for microarray construction

Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.

The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis so you can go straight to qPCR.

In addition, primers for PCR amplification are included to make double-stranded cDNA via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.

Choose the SeraMir Kit that’s right for you

Cat. # Name Kit includes
ExoQuick or ExoQuick-TC SeraMir RNA columns and reagents SeraMir cDNA synthesis and amplification reagents 384-well plate miRNAs for human, mouse, or rat
RA800A-1 Complete SeraMir Exosome RNA Amplification Kit  
RA800TC-1 Complete SeraMir Exosome RNA Amplification Kit for Media and Urine  
RA806A-1 SeraMir Exosome RNA Purification Kit    
RA806TC-1 SeraMir Exosome RNA Purification Kit for Media and Urine    
RA808A-1 SeraMir Exosome RNA Purification Column Kit      
RA820A-1 Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1 Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1 Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1 Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

Go from sample to amplified exoRNAs in a single day

  • Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
  • Purify exoRNAs with SeraMir columns
  • Tail and tag all exoRNAs for qPCR
  • Perform second strand synthesis to generate cDNAs for amplification and T7 IVT

The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.

Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.


Citations

  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017 Jun 1;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017 May 1; 4(2):112-121. PM ID: 28451447
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017 Apr 15;. PM ID: 28411393
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017 Feb 1; 15:163-172. PM ID: 27974246
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017 Jan 1; 25(1):102-110. PM ID: 27883272
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
  • Reza, AM, et al. (2016) Human adipose mesenchymal stem cell-derived exosomal-miRNAs are critical factors for inducing anti-proliferation signalling to A2780 and SKOV-3 ovarian cancer cells. Sci Rep. 2016 Dec 8; 6:38498. PM ID: 27929108
  • Liu, C, et al. (2016) Serum exosomal miR-4772-3p is a predictor of tumor recurrence in stage II and III colon cancer. Oncotarget. 2016 Nov 15; 7(46):76250-76260. PM ID: 27788488
  • Nakamura, K, et al. (2016) Altered expression of CD63 and exosomes in scleroderma dermal fibroblasts. J. Dermatol. Sci.. 2016 Oct 1; 84(1):30-39. PM ID: 27443953
  • Gutierrez, MJ, et al. (2016) Airway Secretory microRNAome Changes during Rhinovirus Infection in Early Childhood. PLoS ONE. 2016 Sep 20; 11(9):e0162244. PM ID: 27643599
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016 Jul 20; 39:427-432. Link: 中华检验医学杂志
  • Yan, IK, et al. (2016) Circulating Extracellular RNA Markers of Liver Regeneration. PLoS ONE. 2016 Jul 15; 11(7):e0155888. PM ID: 27415797
  • Xiao, J, et al. (2016) Cardiac progenitor cell-derived exosomes prevent cardiomyocytes apoptosis through exosomal miR-21 by targeting PDCD4. Cell Death Dis. 2016 Jun 23; 7(6):e2277. PM ID: 27336721
  • Huleihel, L, et al. (2016) Matrix-bound nanovesicles within ECM bioscaffolds. Sci Adv. 2016 Jun 1; 2(6):e1600502. PM ID: 27386584
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016 Mar 2; 11(3):e0150154. PM ID: 26930277