Cas9-expressing HEK293 Cell Line

Get right to gRNA screening, functional validation and more with HEK293 cells that stably express Cas9 from the AAVS1 safe harbor site.

Description
Size
Catalog Number
Price
Quantity
Add to Cart

Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus

Array ( [attributes] => Array ( [attribute_size] => 2 x 10^6 Cells ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 995 [display_regular_price] => 995 [image] => Array ( [title] => cas9-protein [caption] => [url] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein.png [srcset] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein.png 345w, https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein-300x300.png 300w, https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein-100x100.png 100w [sizes] => (max-width: 345px) 100vw, 345px [full_src] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein.png [full_src_w] => 345 [full_src_h] => 345 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/crispr-cas9-systems/cas9-protein-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 345 [src_h] => 345 ) [image_id] => 14157 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $995 [sku] => CAS630A-1 [variation_description] =>

Stable Cas9 HEK293 Cell Line with Cas9 stably integrated at AAVS1 Safe Harbor Locus

[variation_id] => 63302 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
2 x 10^6 Cells
CAS630A-1
$ 995
Contact Us Speak to a specialist
1-888-266-5066

Overview

Get right to genome-wide gRNA screening, high-throughput functional validation, and more

When you’re studying gene function or conducting other screens in HEK293 cells, SBI helps you get right to screening with our Cas9-expressing HEK293 Cell Line. This cell line comes with the hspCas9 gene already integrated into the AAVS1 safe harbor site for robust and stable Cas9 expression.

How It Works

Genome engineering with CRISPR/Cas9

For general guidance on using CRISPR/Cas9 technology for genome engineering, take a look at our CRISPR/Cas9 tutorials as well as the following application notes:

CRISPR/Cas9 Gene Knock-Out Application Note (PDF) »
CRISPR/Cas9 Gene Editing Application Note (PDF) »
CRISPR/Cas9 Gene Tagging Application Note (PDF) »

CRISPR/Cas9 Basics

Through careful selection of the target sequence and design of a donor plasmid for homologous
recombination, you can achieve efficient and highly targeted genomic modification with CRISPR/Cas9.

The system

A quick overview of the CRISPR/Cas9 System.

Cas9 protein—uses guide RNA (gRNA) to direct site-specific, double-strand DNA cleavage adjacent to a protospacer adapter motif (PAM) in the target DNA.

gRNA—RNA sequence that guides Cas9 to cleave a homologous region in the target genome. Efficient cleavage only where the gRNA homology is adjacent to a PAM.

PAM—protospacer adapter motif, NGG, is a target DNA sequence that spCas9 will cut upstream from if directed to by the gRNA.

The workflow at-a-glance

DESIGN: Select gRNA and HR donor plasmids. Choice of gRNA site and design of donor
plasmid determines whether the homologous recombination event results in a knock-out,
knock-in, edit, or tagging.

CONSTRUCT: Clone gRNA into all-in-one Cas9 vector. Clone 5’ and 3’ homology arms into HR
donor plasmid. If creating a knock-in, clone desired gene into HR donor.

CO-TRANSFECT or CO-INJECT: Introduce Cas9, gRNA, and HR Donors into the target cells
using co-transfection for plasmids, co-transduction for lentivirus, or co-injection for mRNAs.

SELECT/SCREEN: Select or screen for mutants and verify.

VALIDATE: Genotype or sequence putative mutants to verify single or biallelic conversion.