Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

Product Filters

Select a filter to display only products matching that category.

MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV125PA-1), 1 Kit (Cat. # CASLV125PA-KIT), 2 x 25 µL (Cat. # CASLV125VA-1)

CMV-hspCas9(D10A)-T2A-Puro Two Vector Cas9 SmartNickaseTM Lentivector

When working with difficult-to-transfect-cells and you want to reduce off-target events, use our lentiviral Cas9 Nickase—CMV-hspCas9(D10A)-T2A-Puro.
Sizes:10 µg (Cat. # CASLV200PA-1), 1 Kit (Cat. # CASLV200PA-KIT), 2 x 25 µL (Cat. # CASLV200VA-1)

CMV-hspCas9(D10A)-EF1α-GFP Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase - CMV-hspCas9(D10A)-EF1α-GFP .
Sizes:10 µg (Cat. # CASLV205PA-1), 1 Kit (Cat. # CASLV205PA-KIT), 2 x 25 µL (Cat. # CASLV205VA-1)

MSCV-hspCas9(D10A)-T2A-Puro Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase—MSCV-hspCas9(D10A)-T2A-Puro.
Sizes:10 µg (Cat. # CASLV220PA-1), 1 Kit (Cat. # CASLV220PA-KIT), 2 x 25 µL (Cat. # CASLV220VA-1)

MSCV-hspCas9(D10A)-EF1α-GFP Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase-MSCV-hspCas9(D10A)-EF1α-GFP .
Sizes:10 µg (Cat. # CASLV225PA-1), 1 Kit (Cat. # CASLV225PA-KIT), 2 x 25 µL (Cat. # CASLV225VA-1)

CMV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNucleaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—CMV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV300PA-1)

MSCV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNucleaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—MSCV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV320PA-1)

CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNickaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and enhanced target specificity with Cas9 nickase - CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV400PA-1)

MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNickaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and enhanced target specificity with Cas9 nickase - MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV420PA-1)

EF1α-Blasticidin-H1-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-Blasticidin-H1-gRNA.
Size:10 Reactions (Cat. # CASLV500PA-B)

EF1α-GFP-H1-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-GFP-H1-gRNA.
Size:10 Reactions (Cat. # CASLV501PA-G)

EF1α-RFP-H1-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System - EF1α-RFP-H1-gRNA.
Size:10 Reactions (Cat. # CASLV502PA-R)

EF1α-Blasticidin-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System - EF1α-Blasticidin-U6-gRNA.
Size:10 Reactions (Cat. # CASLV510PA-B)

CMV-hspCas9-T2A-Puro Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has Puro selection.
Sizes:10 µg (Cat. # CASLV100PA-1), 1 Kit (Cat. # CASLV100PA-KIT), 2 x 25 µL (Cat. # CASLV100VA-1)

EF1α-GFP-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-GFP-U6-gRNA.
Size:10 Reactions (Cat. # CASLV511PA-G)

CMV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV105PA-1), 1 Kit (Cat. # CASLV105PA-KIT), 2 x 25 µL (Cat. # CASLV105VA-1)

EF1α-RFP-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-RFP-U6-gRNA.
Size:10 Reactions (Cat. # CASLV512PA-R)

MSCV-hspCas9-T2A-Puro Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has Puro selection.
Sizes:10 µg (Cat. # CASLV120PA-1), 1 Kit (Cat. # CASLV120PA-KIT), 2 x 25 µL (Cat. # CASLV120VA-1)

CMV-hspCas9-T2A-Puro-H1-AAVS1_gRNA All-in-one Cas9 SmartNucleaseTM & AAVS1 gRNA Lentivector

Streamline genome editing at the AAVS1 Safe Harbor Site in hard-to-transfect cells with a lentivector that expresses both Cas9 and an AAVS1-targeting gRNA
Sizes:10 µg (Cat. # CASLV601PA-1), 2 x 25 µL (Cat. # CASLV601VA-1)