Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

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CMV-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP-CMV-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS741R-1)

CMV-hspCas9-nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid (circular)

Use this first-generation SBI CMV-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector.
Size:10 µg (Cat. # CAS840A-1)

CMV-hspCas9(D10A)-EF1α-GFP Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase - CMV-hspCas9(D10A)-EF1α-GFP .
Sizes:10 µg (Cat. # CASLV205PA-1), 1 Kit (Cat. # CASLV205PA-KIT), 2 x 25 µL (Cat. # CASLV205VA-1)

EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Use the EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS750A-1)

EF1α-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the EF1α promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS900A-1)

MSCV-hspCas9(D10A)-T2A-Puro Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase—MSCV-hspCas9(D10A)-T2A-Puro.
Sizes:10 µg (Cat. # CASLV220PA-1), 1 Kit (Cat. # CASLV220PA-KIT), 2 x 25 µL (Cat. # CASLV220VA-1)

EF1α-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNickase Plasmid that delivers multiple gRNAs—kit bundles EF1α-T7-hspCas9-Nickase-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS750A-KIT)

CAG-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CAG promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS920A-1)

MSCV-hspCas9(D10A)-EF1α-GFP Two Vector Cas9 SmartNickaseTM Lentivector

When you’re working with difficult-to-transfect-cells and want to reduce off-target events, use our lentiviral Cas9 Nickase-MSCV-hspCas9(D10A)-EF1α-GFP .
Sizes:10 µg (Cat. # CASLV225PA-1), 1 Kit (Cat. # CASLV225PA-KIT), 2 x 25 µL (Cat. # CASLV225VA-1)

EF1α-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses GFP-EF1α-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS750G-1)

CMV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the CMV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS940A-1)

CMV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNucleaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—CMV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV300PA-1)

EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RF-EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS751R-1)

MSCV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the MSCV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS960A-1)

MSCV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNucleaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—MSCV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV320PA-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS700A-1)

CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Use the CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS770A-1)

PGK-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the PGK promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS980A-1)

CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNickaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and enhanced target specificity with Cas9 nickase - CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV400PA-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs—kit bundles EF1α-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS700A-KIT)