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phiC31 Integrase
Vector System

One-step gene addition technology

Nonviral transgene delivery

  • Single-copy integration
  • Integrates at active sites
  • Unlimited cargo capacity
  • Make stable cell lines easily

Nonviral transgene delivery

   

One-step gene addition technology
The phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 base pair sequences termed attachment sites (att), one found in the phage and the other in the bacterial host. This serine integrase has been show to function efficiently in many different cell types including mammalian cells. In the presence of phiC31 integrase, an attB- containing donor plasmid can be unidirectional integrated into a target genome through recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable.

Features

  • Nonviral transgenesis
  • Single-copy integration
  • Preferred integration at active transcription sites
  • Unlimited cargo capacity
  • Easily make stable cell lines using transfection-based methods
  • Suitable for engineering Stem Cells and In vivo animal models