What is unique about SBI’s MicroRNA Precursor Clone Collections?

The most comprehensive microRNA Precursor Clone Collection in HIV-based Lentiviral Vectors available anywhere.

There are expected to be over 1,000 microRNAs encoded in the human and mouse genomes and they function by either blocking translation of, or degrading, mRNA species corresponding to specific genes. While the number of verified human microRNAs is expanding, there is an increasing need for effective functional testing. System Biosciences (SBI) is offering an extensive collection of microRNA precursors in lentiviral vectors that can be used to modulate the expression of their targeted mRNAs in vivo, and thereby study microRNA function. The constructs are built in a lentiviral vector backbone, so they can be packaged into lentiviral particles and delivered to primary cells, stem cells, or other hard-to-transfect cell lines.

Each construct in SBI’s collection consists of the native stem loop structure and 200-400 base pairs of upstream and downstream flanking genomic sequence. This unique feature ensures that the microRNAs expressed from SBI’s constructs will be correctly processed in the cell into mature microRNA.

System Biosciences (SBI) is the only commercially-available collection of microRNAs that are cloned in Lentiviral vectors capable of being propagated. The advantage of SBI’s constructs is that they can be stably expressed in a wide variety of cell types, as opposed to synthetic microRNAs that can only be transiently expressed in cells. Moreover, the inserts in the SBI’s microRNA Precursor Clone Collections represent more than just the mature microRNA sequences listed in Sanger’s miRBase (http://www.mirbase.org/search.shtml).

First microRNA Precursor Collection Available in HIV-based Lentiviral Vectors. The precursors express microRNAs several-fold above endogenous microRNA levels.

• List of microRNA precursor clones