Stem Cell Collection
Profile microRNAs in differentiation
Pre-formatted qPCR array
- Stem cell self-renewal
- Hematopoiesis
- Neural development
- Tissue specific microRNAs
Features and Benefits
- Simultaneously profile 95 different MicroRNAs known to be involved in Stem Cell self-renewal, hematopoiesis, neural development and tissue patterning
- Identify MicroRNA development biomarkers and expression pattern signatures in Stem Cells
- System rapidly tags and converts all small RNAs into detectable cDNA for qPCR
- Simple and robust procedure
- Sensitive and quantitative down to picogram amounts of starting total RNA
- Amenable to high-throughput screening of numerous cell lines and tissues
Stem Cells MicroRNA qPCR Array Layout
Convenient 96-well Plate Format, segmented into 4 separate
Stem Cell Pathways
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | miR- 18a |
miR- 19a |
miR- 19b |
miR- 24 |
miR- 25 |
miR- 17-5p |
miR- 30c |
miR- 34a |
miR- 106a |
miR- 106b |
miR- 130b |
miR- 141 |
| B | miR- 150 |
miR- 199a |
miR- 200b |
miR- 200c |
miR- 301 |
miR- 302a |
miR- 302b |
miR- 302c |
miR- 302d |
miR- 367 |
miR- 368 |
miR- 369-5p |
| C | miR- 369-3p |
miR- 370 |
miR- 371 |
miR- 372 |
miR- 373 |
let- 7a |
let- 7b |
miR- 10a |
miR- 10b |
miR- 16 |
miR- 17-3p |
miR- 20a |
| D | miR- 20b |
miR- 23a |
miR- 23b |
miR- 26a |
miR- 26b |
miR- 30b |
miR- 30d |
miR- 32 |
miR- 33 |
miR- 92 |
miR- 93 |
miR- 99a |
| E | miR- 101 |
miR- 107 |
miR- 126 |
miR- 130a |
miR- 142-5p |
miR- 142-3p |
miR- 146a |
miR- 146b |
miR- 155 |
miR- 181a |
miR- 181b |
miR- 181c |
| F | miR- 181d |
miR- 191 |
miR- 193a |
miR- 193b |
miR- 197 |
miR- 221 |
miR- 223 |
miR- 339 |
miR- 9 |
miR- 103 |
miR- 124a |
miR- 125a |
| G | miR- 125b |
miR- 127 |
miR- 128a |
miR- 128b |
miR- 132 |
miR- 134 |
miR- 135a |
miR- 135b |
miR- 136 |
miR- 138 |
miR- 149 |
miR- 153 |
| H | miR- 154 |
miR- 183 |
miR- 218 |
miR- 219 |
miR- 222 |
miR- 1 |
miR- 122a |
miR- 133a |
miR- 133b |
miR- 195 |
miR- 206 |
U6 snRNA |
| Stem Cell Signature Set | |
| Hematopoietic Set | |
| Neuronal Set | |
| Organ Set |
Sample MicroRNA qPCR Panel Data
The Stem Cells microRNA qPCR Panel was used to quantitate microRNAs in Human Cord Blood CD34+ Stem Cells.
Real-time amplification plot
Graphical representation of relative abundance of microRNAs in Human Cord Blood CD34+ Stem Cells (levels Normalized to U6)
Kit Components
|
40µl
|
5x PolyA polymerase Buffer |
|
10µl
|
PolyA polymerase |
|
20µl
|
25 mM MnCl2 |
|
30µl
|
5 mM ATP |
|
10µl
|
3' Oligo dT Adaptor |
|
80µl
|
5x Reverse Transcriptase Buffer |
|
20µl
|
Reverse Transcriptase |
|
30µl
|
0.1 M Dithiothreitol (DTT) |
|
50µl
|
dNTP Mix |
|
600µl
|
3' Universal Reverse PCR Primer |
+ One 96-well Stem Cells microRNA Primer stock plate (enough for 10 entire panel measurements)
Kits should be stored at -20oC upon receipt.