ExoQuick™, ExoQuick-TC™ and ExoQuick-LP™
Polymer-based exosome precipitation
One-step Exosome Isolation
- Serum and Plasma
- Tumor Ascites Fluid
- Spinal fluid
- Tissue Culture Media
Need exosomes? SBI's ExoQuick products enable high-throughput, quantitative isolation of exosomes from low sample volumes (as little as 100µl serum). Compatible with any biofluid and a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation (refs 1-3).
ExoQuick is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
Exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation in electron microscopy studies (refs 1,2), and are active in numerous functional assays (refs 2,3) Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models. ExoQuick exosome isolation methods are patented technologies (ref 4).
"We therefore pursued the Exoquick method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models."(ref 2).
The ExoQuick Family
ExoQuick for most biofluids
ExoQuick-TC for tissue culture and urine
ExoQuick-LP to remove contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation
1. Umezu T, Ohyashiki K, Kuroda M, Ohyashiki JH. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2012 Jul 16. doi: 10.1038/onc.2012.295. (PDF) »
2. Chugh PE, Sin S-H, Ozgur S, Henry DH, Menezes P, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog 9(7): e1003484. doi:10.1371/journal.ppat.1003484.
3. Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4;8(11):e78505.
4. Antes, T et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.