ExoQuick® Family of Reagents
Polymer-based exosome precipitation
One-step Exosome Isolation
- Serum and Plasma
- Spinal fluid
- Tissue Culture Media
The ExoQuick Family
ExoQuick for plasma, serum, and ascites fluid
ExoQuick-TC for all other biofluids
ExoQuick-CG for pre-clinical in vivo applications
ExoQuick-LP to remove contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation
ExoQuick® PLUS & ExoQuick-TC® PLUS for sensitive applications such as mass spectrometry, exosome labeling, and in vivo/ex vivo exosome delivery
Need exosomes? SBI's ExoQuick products enable high-throughput, quantitative isolation of exosomes from low sample volumes (as little as 100µl serum). Compatible with any biofluid and a wide variety of downstream applications, ExoQuick is an effective and proven alternative to ultracentrifugation (1-3).
ExoQuick is a proprietary polymer that gently precipitates exosomes. First, pre-clear your samples of cells and cellular debris, and then simply add the appropriate amount of ExoQuick to your cleared biofluid, refrigerate, and centrifuge (see the product manual for protocol details). Your exosomes will be in the pellet, ready for resuspension in an appropriate solution.
Exosomes isolated with ExoQuick appear similar to exosomes isolated using ultracentrifugation in electron microscopy studies (1,2), and are active in numerous functional assays (2,3). Backed by a growing number of publications, ExoQuick is often the best option for researchers working with low sample volumes, such as clinical research samples or small animal models. ExoQuick exosome isolation methods are patented technologies (4).
"We therefore pursued the Exoquick method for further study, as these samples required much less sample input, a key benefit when working with clinical samples and mouse models."(2).
ExoQuick Workflows - High-throughput, Quantitative Exosome Recovery
ExoQuick can be used to purify exosomes from a wide variety of biofluids, including saliva (5), urine (6), follicular fluid (7), plasma (8), serum (9), tissue culture media (10), breast milk (11), and malignant ascites (12). With a simple workflow involving minimal hands-on time and low input sample volume requirements, ExoQuick is an excellent option for researchers who need to purify multiple exosome samples and/or samples from small animal models or clinical research samples.
Example - Exosome Isolation from Serum
To isolate exosomes from cleared serum, simply add an appropriate volume of ExoQuick to as little as 100 µl serum. Incubate for at least one hour at 4°C, then isolate exosomes with a 30 minute low-speed spin (1500 x g). Isolated exosomes can be found in the pellet, and resuspended in an appropriate solution.
You can verify the presence of exosomes with a number of different methods, including Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70), NanoSight analysis, or EM (learn more about different ways to detect and quantify exosomes). Here we show an example of Western blot data using exosomes isolated from 250µl serum. Small RNAs were also extracted using Trizol from exosomes isolated from 5 ml serum for visualization on a sybrGold-stained 15% polyacrylamide gel. Only the ExoQuick Pellet contained small RNAs.
With ExoQuick, you can obtain high-quality exosomes from most biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample.
1. Umezu T, Ohyashiki K, Kuroda M, Ohyashiki JH. Leukemia cell to endothelial cell communication via exosomal miRNAs. Oncogene. 2012 Jul 16. doi: 10.1038/onc.2012.295. (PDF) »
2. Chugh PE, Sin S-H, Ozgur S, Henry DH, Menezes P, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog 9(7): e1003484. doi:10.1371/journal.ppat.1003484.
3. Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4;8(11):e78505.
4. Antes, T et al. Methods for Microvesicle Isolation and Selective Removal. Patent No.: US 9,005,888 B2.
5. Yang J, Wei F, Schafer C, Wong DTW. Detection of Tumor Cell-Specific mRNA and Protein in Exosome-Like Microvesicles from Blood and Saliva. PLoS ONE 9(11): e110641.
7. Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D. Exosomal and Non-Exosomal Transport of Extra-Cellular microRNAs in Follicular Fluid: Implications for Bovine Oocyte Developmental Competence. PLoS One. 2013 Nov 4;8(11):e78505.
8. Chugh PE, Sin S-H, Ozgur S, Henry DH, Menezes P, et al. Systemically Circulating Viral and Tumor-Derived MicroRNAs in KSHV-Associated Malignancies. PLoS Pathog 9(7): e1003484. doi:10.1371/journal.ppat.1003484.
9. Epple LM, Griffiths SG, Dechkovskaia AM, Dusto NL, White J, Ouellette RJ, Anchordoquy TJ, Bemis LT, Graner MW. Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles. (2012) PLoS ONE 7(7): e42064. doi:10.1371/journal.pone.0042064. (PDF) »
10. Lin Zhu, Xiu-Hua Qu, Yu-Lin Sun, Yang-Ming Qian and Xiao-Hang Zhao. Novel method for extracting exosomes of hepatocellular carcinoma cells. World J Gastroenterol. 2014 June 7; 20(21): 6651-6657.
11. Gu Y, Li M, Wang T, Liang Y, Zhong Z, et al. Lactation-Related MicroRNA Expression Profiles of Porcine Breast Milk Exosomes. (2012) PLoS ONE 7(8): e43691. doi:10.1371/journal.pone.0043691. (PDF) »
12. As featured in: Exosome Isolation for Proteomic Analyses and RNA Profiling Douglas D. Taylor, Wolfgang Zacharias and Cicek Gercel-Taylor, Serum/Plasma Proteomics, Methods in Molecular Biology, 2011, Volume 728, Part 4, 235-246, (PDF) »