Global MicroRNA
Amplification & Cloning Kit
Amplify and clone mature microRNAs
Rapidly clone mature microRNAs
- Ligation for first strand synthesis
- MicroRNAs tagged at both ends
- Obtain excellent representation
Online Resources
Product Citations
Leticia Frizzo da Silva and Clinton Jones. Two MicroRNAs Encoded within the Bovine Herpesvirus 1 Latency-Related Gene Promote Cell Survival by Interacting with RIG-I and Stimulating NF-kB-Dependent Transcription and Beta Interferon Signaling Pathways. J. Virol. 2012, 86(3):1670. (PDF) »
Jaber T, Workman A, Jones C. 2010. Small non-coding RNAs encoded within the bovine herpesvirus 1 latency related gene can reduce steady state levels of infected cell protein 0 (bICP0). J Virol. 2010 Apr 21.
Benchun Liu, Gerald R. Cunha, Laurence S. Baskin. (2009). Differential expression of microRNAs in mouse embryonic bladder. Biochemical and Biophysical Research Communications, Volume 385, Issue 4, 7 August 2009, Pages 528-533. (PDF) »
Tesfaye D, Worku D, Rings F, Phatsara C, Tholen E, Schellander K, Hoelker M. (2009). Identification and expression profiling of microRNAs during bovine oocyte maturation using heterologous approach. Mol Reprod Dev. 2009 Jul;76(7):665-77. (PDF) »
Weiping Peng, Olga Vitvitskaia, Dale Carpenter, Steven L. Wechsler, Clinton Jones. 2008. Identification of two small RNAs within the first 1.5-kb of the herpes simplex virus type 1-encoded latency-associated transcript. Journal of Neurovirology, Volume 14, Issue 1, pages:41–52.
Technical References
Sonthelmer, E. J., Carthew, R. W. 2005. Silence from within: Endogenous siRNAs and miRNAs. Cell 122:9-12.
Zamore, P.D., Haley, B. 2005. Ribo-gnome: The big world of small RNAs. Science 309: 1519-1524.
Bartel, D. 2004. MicroRNAs: Genomics, Biogenesis, Mechanism, and Function. Cell 116: 281-297.
Kim, Narry V. 2005.Small RNAs: Classification, Biogenesis, and Function. Mol. Cells. 19:1-15.
Valencia-Sanchez, MA., Liu, J., Hannon, GJ., Parker, R., 2006. Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev 20: 515-525.
Lewis B.P, Burge C.B, Bartel, D.P. 2005. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120: 15-20.
Xie X., Lu J., Kulbokas, E.J., Goulub, T.R., Mooth, V., Lindblad-Toh, K., Lander, E.S. and Kellis, M. Systematic discovery of regulatotory motifs in human promoters and 3’ UTRs by comparison of several mammals. Nature.434:338-45.
Lagos-Quintana, M., Rauhut, R., Lendeckel, W., Tuschl, T. 2001. Identification of Novel Genes Coding for Small Expressed RNAs. Science 294: 853-858.
Basyuk, E., Suavet, F., Doglio, A., Bordonne, R., Bertrand, E. 2003. Human let-7 stem-loop precursors harbor features of RNase III cleavage products. Nucleic Acids Res 31: 6593-6597.
Shi, R., Chiang, V.L., 2005. Facile means for quantifying microRNA expression by real-time PCR. BioTechniques. 39:519-525.
Ding, Y., Chan, C.Y., and Lawrence, C.E. (2005) RNA secondary structure prediction by centroids in a Boltzmann weighted ensemble. RNA 11, 1157-1166.
Griffiths-Jones,S., Grocock, R.J., Van Dongen, S., Bateman, A., Enright, A.J. 2006. miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Research 34: D140-D144.
Shingara, J., Keiger, K., Shelton, J., Laosinchai-Wolf, W., Powers, P., Conrad, R., Brown, D., Labourier, E. 2005. An optimized isolation and lebeling platgorm for accurate microRNA expression profiling. RNA 11:1461-1470.
He, L., Thomson, J.M., Hemann, M.T., Hernando-Monge, E., Mu, D., Goodson, S., Powers, S., Cordon-Cardo, C., Lowe, S.W., Hannon, G.J., Hammond, S.M. 2005. A microRNA polycistron as a potential human oncogene. Nature 435: 828-833.
Lai, E.C., Wiel, C., Rubin, G.M. 2004. Complementary miRNA pairs suggest a regulatory role for miRNA:miRNA duplexes. RNA 10:171-175.
Ambros, V., Bartel, B., Bartel, D.P., Burge, C.B., Carrington, J.C., Chen, X., Dreyfuss, G., Eddy, S.R., Griffiths-Jones, S., Marshall, M., Matzke, M., Ruvkun, G., Tuschl, T. 2003. A uniform system for microRNA annotation. RNA 9:277-279.
Obernosterer, G., Leuschner, P.J.F., Alenius, M., Martinez, J. 2006. Post-transcriptional regulation of microRNA expression. RNA 12:1-7.
Dostie, J., Mourelatos, Z., Yang, M., Sharma, A., Dreyfuss, G. 2003. Numerous microRNPs in neuronal cells containing novel microRNAs. RNA 9: 180-186.



