The LncProfiler qPCR Array Kits

• Human Arrays: catalog#s RA900A-1 and RA910A-1
• Mouse Array: catalog# RA930A-1

SBI's LncProfilers come as complete cDNA synthesis kits combined with a 96-well based qPCR assay Human or Mouse assay sets to quantitate 90 different lncRNAs, 5 positive housekeeping reference controls and one negative control. The qPCR assays have been validated across numerous cell types for robust and specific performance. Some lncRNAs have endogenouse polyA tails, while other lncRNAs do not. To enhance qPCR assay performance, the cDNA synthesis kit includes reagents to polyadenylate all lncRNAs before cDNA conversion using the tagged oligo dT adaptor and random primers. This combined RNA tailing and oligo dT plus random primers boosts cDNA yield significantly and enables strand-specific lncRNA qPCR profiling.

Kit components for catalog#s RA900A-1 and RA930A-1

• 5X PolyA Polymerase Buffer, • PolyA Polymerase
• 25 mM MnCl2, • 5 mM ATP, • Oligo dT Adaptor
• 5X Reverse Transcriptase Buffer + Random Primer Mix
• Reverse Transcriptase, • 0.1 M Dithiothreitol (DTT), • dNTP Mix
• Array Primers, dried down in Primer plate

Optional for catalog# RA910A-1 kit with SYBR Green for first 2 profiles

• 2X SYBR Green master Mix

Subcellular reference controls

LncRNAs can localize and function in the nucleolus, nucleus and in the cytoplasm. The LncProfiler qPCR array includes RNA reference controls to allow for subcellular fractionation studies to identify and profile three separate subcellular compartments.
• Nucleolus: snoRNA RNU43 (and some 18S rRNA)
• Nucleus: small Nuclear splicing snRNA U6B
• Cytoplasm: GAPDH, Lamin A/C (Human Array) and Beta Actin (Mouse Array) as well as 18S rRNA