Non-coding RNA qPCR Arrays
Quantitate non-coding RNAs
by real-time qPCR
Profile ncRNAs in Cancer, Stem Cells and Disease
- Sensitive and Specific Assays
- LncRNAs involved in epigenome changes
- Regulatory RNAs involved in transcription control
Long noncoding RNAs (LncRNAs) are emerging as master regulators of embryonic pluripotency, differentiation, patterning of the body axis and promoting developmental transitions. LncRNAs are larger than 200 nucleotides in length and are pervasively expressed across the genome. LncRNAs function as molecular scaffolds regulating histone modifications and influence the epigenetic programs of the transcriptome. Dysregulated expression of lncRNAs has been shown to be associated with a broad range of defects in development and diseases. Studying the expression patterns of lncRNAs will be a crucial method to understanding the roles they play in many model systems. All of the lncRNAs on the LncProfiler™ qPCR array have validated primer sets for well-annotated lncRNAs that are registered in the lncRNA database created by Dr. John Mattick.
Functions of lncRNAs
To date, lncRNAs have been found to exhibit a wide range of functions ranging from signaling, serving as molecular decoys, guiding ribonulceoprotein complexes to specific chromatin sites and also participating as scaffolds in the formation of complexes.
The transcription of certain lncRNAs is very tissue and temporal specific. Their expression can be in response to certain stimuli, such as cellular stress and temperature. Thus, lncRNAs can serve as molecular signals and can act as markers of functionally significant biological events.
The molecular decoy type of activity takes place when specific lncRNAs are transcribed and then bind to and titrate away protein factors. Decoy lncRNAs can "sponge" protein factors such as transcription factors and chromatin modifiers. This leads to broad changes in the cell's transcriptome.
LncRNAs can be molecular guides by localizing particular ribonucleoprotein complexes to specific chromatin targets. This activity can cause changes in gene expression either in cis (on neighboring genes) or in trans (distantly located genes) that cannot be easily predicted by just the lncRNA sequence itself.
Assembly of complex protein complexes can be supported by lncRNAs, linking factors to together to form new functions. Some lncRNAs possesses different domains that bind distinct protein factors that altogether, may impact transcriptional activation or repression.
Graphic at right adapted from: Wang, KC and Chang HY, Molecular Mechanisms of Long Noncoding RNAs. Mol Cell. 2011 Sep 16;43(6):904-14.