Non-coding RNA qPCR Arrays
Quantitate non-coding RNAs
by real-time qPCR
Profile ncRNAs in Cancer, Stem Cells and Disease
- Sensitive and Specific Assays
- LncRNAs involved in epigenome changes
- Regulatory RNAs involved in transcription control
Disease-related human lncRNA Profiler™
Long noncoding RNAs (lncRNAs), as a new class of transcripts, have been recently revealed to be pervasively transcribed in the genomes of human and mouse. Multiple lines of evidence increasingly link lncRNAs to diverse human diseases. Disease-related human lncRNA profiler qPCR array allows for the quantification of differential expression of 83 individual lncRNAs among various experimental RNA samples. All 83 lncRNAs chosen for the array are based on publications and they are implicated in diseases ranging from neurodegeneration to cancer. The array plate also includes the house keeping genes and small RNA transcripts for normalization purposes. Using real-time RT-PCR, you can easily and reliably analyze expression of a panel of lncRNAs that have potential roles in a variety of cellular processes such as cell cycle progression, differentiation, self-renewal and apoptosis. Disease-related human lncRNA profiler is easy, convenient, sensitive and specific. It is suitable for cDNA synthesized by either random primer, oligo dT primer, or strand specific primer. It can be used for research in cancer, stem cells, immunology, biomarker discovery & validation, as well as phenotypic analysis of cells.
LncRNAs participate in a wide variety of biological processes
Recent examples of mutated lncRNAs implicated in disease include ANRIL and HOTAIR that bind to chromatin-remodeling complexes PRC1 and PRC2 to alter chromatin and transcription. GAS5 lncRNA acts as a decoy for the GR transcription factor and prevents GR from binding to DNA and transcriptional activation. MALAT1 RNA binds to SR proteins to regulate mRNA alternative splicing, whereas BACE-1AS RNA binds to the complementary BACE-1 mRNA to regulate BACE-1 translation. Graphic and legend adapted from: Orly Wapinski and Howard Y. Chang, Long noncoding RNAs and human disease. Trends Cell Biol. 2011 Jun;21(6):354-61.