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PrecisionX™ HR Targeting Vectors

Genome engineering with
homology-directed recombination vectors

HR Targeting Vectors

  • Dual Marker Options
  • Built-in Insulators
  • LoxP Sites for Removal
  • HR-arm Multiple Cloning Sites

PrecisionX™ HR Targeting Vectors

Recent advances in tools available for precise genome engineering of target cells have revolutionized the field of biology. Transcription activator- like effector nucleases (TALENs), first described in the plant pathogen Xanthomonas sp., have shown that researchers can efficiently target any genomic DNA sequence using a pair of custom TALEN proteins whose DNA-binding modules can recognize individual DNA nucleotides based on an elegant amino acid cipher (Boch et al. 2009). Based on the explosion of recent publications that have successfully demonstrated the utility of the TALEN platform in many eukaryotic model organisms ranging from zebrafish to humans, it has quickly established itself as the de facto platform for genome engineering.

   

However, in order to fully leverage the powerful nature of these genome engineering platforms, there needs to be a set of ancillary tools that will allow detection of cells that have been modified by TALEN or Cas9 systems. The majority of genome editing events affect only a percentage of the total population of transfected cells, estimated at 1-80% for mono- or bi-allelic modifications depending on the platform, the cell type, and the DNA target of interest. Due to the wide variation in activity, performing downstream phenotypic assays in a background of wild-type cells is challenging, especially if the phenotype in question is subtle or difficult to discern. Therefore, tools in the form of donor or targeting vectors that contain 1) fluorescent or antibiotic selection markers and 2) a gene fragment of interest to knock-in, knock-out, or correct a wild-type sequence will be extremely useful for obtaining a homogeneous population of cells whose genomes have been successfully targeted. SBI has generated a suite of homologous recombination targeting vectors suitable for the purpose of engineering both protein coding and non-coding genes including microRNAs and LncRNAs genomic loci.