PinPoint site targeting

Once the PinPoint attP site has been successfully placed into the desired genomic site, this site can then be efficiently and routinely targeted for site-specific integration of expression cassettes at that locus. The expression cassette can be driven by EF1a (Cat# PIN500A-1), CAGs (Cat# PIN510A-1), or a custom-designed promoter of your choice (Cat# PIN520A-1). Once the gene or reporter of interesthas been cloned into the PinPoint donor vector, a simple co-transfection of the placed attP site cell line with the PinPoint integrase vector (Cat# PIN200A-1) will result in a specific recombination reaction between the PinPoint attP site and the attB site in the donor vector construct.

PinPoint integrase and donor vectors