phiC31 integrase vector formats

Dual-direction promoter vectors The dual promoter phiC31 vectors feature a dual-direction formats are available in two formats. The pFC-EF1-MCS- PGK-RFP-Puro (cat# FC550A-1) vector has EF1a driving your gene's expression cloned into the MCS and PGK expressing the RFP-Puro combo markers.
The alternative format in pFC-PGK-MCS-EF1-GFP-Puro (cat#FC551A-1) utilizes PGK to express your gene cloned into the MCS and has EF1a driving the GFP-Puro combo markers. Both PGK and EF1a promoters are well-validated to have a broad range of activity across many cell types, including stem cells.

Single promoter and promoterless vector options
The pFC-CMV-MCS-SV40-Kan/Neo phiC31 vector features CMV driving the expression of your gene cloned into the MCS. pFC-CMV-GFP-SV40-Kan/Neo phiC31 vector features CMV driving the expression of GFP as a positive control construct. Downstream is an SV40 promoter expressing the combo Kan/Neo marker for propagation in bacteria with kanamycin and also neomycin selection in mammalian cells.
Promoterless vector format
The pFC-MCS-SV40-Kan/Neo phiC31 integrase vector (cat# FC500A-1) is an entry-level empty vector to allow any promoter and gene cassette to be inserted at the MCS site. This vector alos has the downstream SV40 promoter expressing the combo Kan/Neo marker for propagation in bacteria with kanamycin and also neomycin selection in mammalian cells.

phiC31 integrase expression construct

   

All of the phiC31 donor vectors require co-transfection with the phiC31 integrase expression vector to catalyze the donor vector integration (cat#FC200A-1). This construct has a codon-optimized phiC31 cDNA expressed by CMV for transient expression of the integrase to inert the donor expression vectors into pseudo attP genomic sites using the attB sequences in the donor vectors. This construct has a kanamycin marker for propagation in bacteria.