phiC31 Integrase
Vector System
One-step gene addition technology
Nonviral transgene delivery
- Single-copy integration
- Integrates at active sites
- Unlimited cargo capacity
- Make stable cell lines easily
Online Resources
Technical References
Karow M, Chavez CL, Farruggio AP, Geisinger JM, Keravala A, Jung WE, Lan F, Wu JC, Chen-Tsai Y, Calos MP. Site-specific recombinase strategy to create induced pluripotent stem cells efficiently with plasmid DNA. Stem Cells 29(11):1696-704.
Chalberg TW, Portlock JL, Olivares EC, Thyagarajan B, Kirby PJ, Hillman RT, Hoelters J, Calos MP. Integration specificity of phage phiC31 integrase in the human genome. J Mol Biol. 2006 Mar 17;357(1):28-48.
Lan F, Liu J, Narsinh KH, Hu S, Han L, Lee AS, Karow M, Nguyen PK, Nag D, Calos MP, Robbins RC, Wu JC. Safe genetic modification of cardiac stem cells using a site-specific integration technique. Circulation. 2012 Sep 11;126(11 Suppl 1):S20-8.
Hillman RT, Calos MP. Site-specific integration with bacteriophage phiC31 integrase. Cold Spring Harb Protoc. 2012 May 1;2012(5).
Chavez CL, Keravala A, Chu JN, Farruggio AP, Cuellar VE, Voorberg J, Calos MP. Long-term expression of human coagulation factor VIII in a tolerant mouse model using the phiC31 integrase system. Hum Gene Ther. 2012 Apr;23(4):390-8.
Woodard LE, Keravala A, Jung WE, Wapinski OL, Yang Q, Felsher DW, Calos MP. Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma. PLoS One. 2010 Jun 29;5(6):e11367.