EZ-Genotyping™ Kit
- Mitigates cross-contamination with a single-tube assay
- Delivers robust, reliable results Includes positive controls Successfully amplifies multiple targets on different chromosomes
- Includes positive controls
- Successfully amplifies multiple targets on different chromosomes
- Is easy-to-use—no need for overnight digestion or column purification of genomic DNA
Products
Overview
Overview
Fast, streamlined genotyping
Determine the genotypes of target alleles in most mammalian samples using our EZ-Genotyping™ Kit. Based on a robust endpoint PCR reaction, the EZ-Genotyping Kit mitigates the risk of sample cross-contamination occurring in the endpoint PCR reaction by conducting the genomic DNA extraction in a single tube.
The Kit is compatible with high throughput assays and can be used for a range of applications including identification of genotypes in mixed/clonal cell populations, DNA fingerprinting, and cell identity/contamination analysis.
The complete Kit contains enough reagents to analyze genomic DNA from 50 samples.
- Mitigates cross-contamination with a single-tube assay
- Delivers robust, reliable results
- Includes positive controls
- Successfully amplifies multiple targets on different chromosomes
- Is easy-to-use—no need for overnight digestion or column purification of genomic DNA
- Provides almost everything you need—the Kit contains both genomic DNA template preparation reagents and PCR Master Mix
- Is economical—the Kit contains enough reagents to analyze 50 samples
How It Works
How It Works
The workflow at-a-glance
- Extract genomic DNA
- Wash cells
- Add CellExtract Solution
- Heat for 2 minutes
- Spin for 2 minutes
- Set up and run EZ-Genotyping PCR reaction
- Analyze on an agarose gel
Supporting Data
Supporting Data
The EZ-Genotyping Kit confirms mono- and bi-allelic genomic modifications
The EZ-Genotyping Kit enables the determination of allele status (wild-type (WT), single-allele knock-out (KO), or bi-allelic KO) in genome editing assays using homologous recombination (HR) targeting vectors along with TALENs or Cas9 SmartNucleases. Figure 1 shows the results from ten candidate cell lines that underwent gene knock-outs using TALENs and an HR vector. The genotyping results clearly show the allele status of each of the candidates: WT—Lines 1 and 3; mono-allelic KO—Lines 4, 5, 6, 7, 8, 9 and 10; bi-allelic KO: Line 2.
Figure 1. The EZ-Genotyping Kit confirms mono- and bi-allelic genomic modifications after gene knock-out.
Resources
Citations
Related Products
Products
Overview
Overview
Fast, streamlined genotyping
Determine the genotypes of target alleles in most mammalian samples using our EZ-Genotyping™ Kit. Based on a robust endpoint PCR reaction, the EZ-Genotyping Kit mitigates the risk of sample cross-contamination occurring in the endpoint PCR reaction by conducting the genomic DNA extraction in a single tube.
The Kit is compatible with high throughput assays and can be used for a range of applications including identification of genotypes in mixed/clonal cell populations, DNA fingerprinting, and cell identity/contamination analysis.
The complete Kit contains enough reagents to analyze genomic DNA from 50 samples.
- Mitigates cross-contamination with a single-tube assay
- Delivers robust, reliable results
- Includes positive controls
- Successfully amplifies multiple targets on different chromosomes
- Is easy-to-use—no need for overnight digestion or column purification of genomic DNA
- Provides almost everything you need—the Kit contains both genomic DNA template preparation reagents and PCR Master Mix
- Is economical—the Kit contains enough reagents to analyze 50 samples
How It Works
How It Works
The workflow at-a-glance
- Extract genomic DNA
- Wash cells
- Add CellExtract Solution
- Heat for 2 minutes
- Spin for 2 minutes
- Set up and run EZ-Genotyping PCR reaction
- Analyze on an agarose gel
Supporting Data
Supporting Data
The EZ-Genotyping Kit confirms mono- and bi-allelic genomic modifications
The EZ-Genotyping Kit enables the determination of allele status (wild-type (WT), single-allele knock-out (KO), or bi-allelic KO) in genome editing assays using homologous recombination (HR) targeting vectors along with TALENs or Cas9 SmartNucleases. Figure 1 shows the results from ten candidate cell lines that underwent gene knock-outs using TALENs and an HR vector. The genotyping results clearly show the allele status of each of the candidates: WT—Lines 1 and 3; mono-allelic KO—Lines 4, 5, 6, 7, 8, 9 and 10; bi-allelic KO: Line 2.
Figure 1. The EZ-Genotyping Kit confirms mono- and bi-allelic genomic modifications after gene knock-out.