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SBI’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, our family of ExoGlow labeling reagents improves your ability to track and localize EVs. Our different classes of reagents specifically label EV mRNAs, proteins, and membranes with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate EV studies.

Which ExoGlow Labeling Kit is right for you?

 ExoGlow RNAExoGlow ProteinExoGlow MembraneExoGlow-NTA
UseLabel internal exosomal mRNA content Selectively and covalently label internal EV protein cargoSelectively label intact exosomes to track them in real timeAccurately measure true particle counts vs. traditional light-scattering NTA
Color and Excitation/EmissionGreen (485/537 nm)Red (573/588 nm), Green (511/525 nm), Blue (403/454 nm)Red (465/635 nm)488 nm
Length of Protocol 30-60 minutes 30-60 minutes 30-60 minutes 30-60 minutes
Input Amount 200-500 ug protein equivalent200-500 ug protein equivalent50-100 ug protein equivalent1-100 ug protein equivalent
# of Reactions10202510

Kit Highlights

  • Specific—carefully developed to generate a robust signal specific for internal EV proteins, leading to very low levels of background
  • Compatible—deliver robust performance on EVs isolated using all methods tested—including ExoQuick, ultracentrifugation, and column-based workflows
  • Easy-to-use—labeling protocol is quick and straightforward
  • Powerful—can be used with as little as 50ug of EV’s

 

Imaging EVs with ExoGlow-RNA
ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells


Figure 1. ExoGlow-RNA enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-RNA and followed uptake by HEK293T cells. The fluorescence signal localizes to perinuclear and cytoplasmic regions, as would be expected for mRNA.

Imaging EVs with ExoGlow-Protein

ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells


Figure 2. ExoGlow-Protein enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Protein and followed uptake by HEK293T cells. The punctate fluorescence signal shows internalization of labeled EVs by the target cells.

Imaging EVs with ExoGlow-Membrane

ExoGlow-Membranes enables clear visualization of labeled EVs being internalized by target cells


Figure 3. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells. We labeled HEK293T EVs with ExoGlow-Membrane and followed uptake by HEK293T cells. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.

Imaging EVs using Fluorescent NTA

ExoGlow-NTA demonstrates that conventional NTA overestimates EV concentration in samples irrespective of EV isolation method.


Figure 4. ExoGlow-NTA demonstrates that conventional NTA overestimates EV concentration in samples irrespective of EV isolation method. Representative data comparing conventional NTA and fluorescent NTA for EVs isolated using (A) ExoQuick (10 µg serum protein), (B) ultracentrifugation and wash (1 µg serum protein), or (C) column-based isolation (1 µg serum protein), shows just how much of the conventional NTA signal is due to non-EV particles.