Load small molecule drugs into exosomes for delivery into cells

   

Small moleculae drugs can also be Exo-Fected into exosomes for delivery to target cells. SBI's cumate system features a cumate switch promoter that is highly suppressed by its cognate CymR repressor protein abd can switch on with the addition of the small moleucle inducer, cumate. We tested the loading of cumate into exosomes using the Exo-Fect system and then added those exosomes to cells containing a cumate switch GFP reporter construct. The control exosomes were untransfected exosomes and the cumate-transfected exosomes were treated with Exo-Fect and 450 ug cumate solution. The experimental approach and reporter HEK293 cell data are shown below. The Exo-Fect technology is able to successfully load small molecule drugs into exosomes that are then able to deliver the drug to target cells.