Transfect siRNAs and microRNAs directly into Exosomes

A Texas-Red end-labeled siRNA (20 pmol) was transfected into exosomes using Exo-Fect. The far left panels show the transfection of the fluorescently labeled siRNA into exosomes that were then immunocaptured on CD63 magnetic beads. The right panels show the transfection of unlabeled siRNA into exosomes that were then immunocaptured on CD63 magnetic beads. The magnetic beads were imaged after overnight capture of transfected HEK293 exosomes on CD63 immunobeads (catalog# EXOFLOW32A-CD63).

The transfected, CD63-captured exosomes were eluted from the beads and then added to HEK293 cells grown in exosome-depleted media (catalog# EXOFBS-50A-1). The transfected exosomes were allowed to dock and interact with the cells for 1-2 days and then the cells were imaged to monitor siRNA cargo delivery.