Transfect mRNAs and Plasmid DNA directly into Exosomes

ExoFect kits work with longer RNAs, like mRNA. We transfected 1 ug of an mRNA encoding RFP into exosomes. These exosomes were then added to HEK293 cells and imaged for RFP protein production after 24 hours. Exo-Fect also enables the transfection of plasmid DNA into exosomes. We used 5 ug of plasmid DNA encoding a GFP gene, transfected exosomes and then added to HEK293 cells. The cells were imaged for GFP protein presence after 48 hours. The image data are shown below.