EV Functional siRNA Delivery

Human EV shuttles can knockdown Mouse genes. Human HEK 293 EV shuttles were loaded with either an anti-CD81 siRNA or a Non-targeting siRNA (NT)and then added to Mouse RAWS 264.7 cells. The cell lysates were collected after 18 hours for Western blot analysis of CD81 protein knockdown. Shown to the left, the Human EV shuttles carrying the anti-CD81 siRNA were able to knockdown the expression of the target cell's CD81 protein levels by at least 50% when compared to the Non-targeting siRNA control (NT). The Western image data were quantitated and graphed in the adjacent panel to the right.