EV Delivery Data

Mouse and Human EV shuttles efficiently deliver siRNA to hard-to-transfect cells. JAWS II mouse dendritic cell exosomes were Exo-Fected with 100 pmol Texas-Red conjugated siRNA (non-targeting control included in kits). The siRNA-loaded EV shuttles were then added to naive mouse monocyte macrophage cells (RAWS 264.7) in culture. The cells were imaged after 18 hours and delivery was observed as soon as 4 hours after adding the EV shuttles. More than 80% of the recipient RAWS 264.7 cells internalized the EV shuttle siRNA cargo (lower left set left panels). Human HEK 293 EV shuttles were treated with SBI's Exo-Green kit (cat# EXOG200A-1) which labels exosome internal proteins fluorescently green. The labeled Human EV shuttles were then added to Mouse embryonic stem cells and imaged for cargo delivery after 18 hours. The Human EV shuttles were well-tolerated and the EVs were taken up efficiently by the Mouse embryonic stem cells (lower left set right panels).

How do EV shuttles compare to Lipfectamine® transfection?

Standard Lipofectamine® 2000 transfection protocols were also compared to Human EV shuttles on RAWS 264.7 cells using the exact same amount of Texas-Red labeled siRNA (100 pmol, see comparison data below). EV shuttles are more efficient at transfecting siRNA into transfection-resistant cells in culture.