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CLOuD9 Gene Expression Regulation System

Study the dynamic effects of chromatin structure on gene expression—direct site-specific chromatin loop formation

The CLOuD9 System

  • Site-specific chromatin loops
  • Inducible, reversible loop formation
  • Easy CRISPR/Cas9 targeting
  • Well-validated system

Explore a new frontier in the study of gene expression regulation

Available only from SBI and featured in a recent Nature Communications article1, our CLOuD9 Gene Expression Regulation Kit gives you an exciting and innovative tool for studying—and potentially controlling—gene expression regulation by putting you in charge of chromatin loop formation. Gain a clearer understanding of the dynamic effects of chromatin restructuring on the expression of your gene-of-interest and increase gene expression in certain cellular contexts. Combining the simplicity of CRISPR/Cas9 targeting—free from DNA cleavage activity—with inducible and reversible protein dimerization partners, the CLOuD9 Gene Expression Regulation Kit adds a new dimension to your gene regulation studies.

CLOuD9 for Chromatin Loop Reorganization using CRISPR-dCas9:

  • Innovative—leverage the simplicity of CRISPR/Cas9 targeting—free of DNA cleavage activity—for inducible and reversible chromatin loop formation anywhere in the genome
  • Well-validated—chromatin loop formation and the resulting changes in gene expression across multiple loci and cell lines is amply demonstrated in Morgan, et al,1
  • Broadly applicable—great for studying the role of chromatin in the control of gene expression in a range of areas, including:
    • Developmental biology
    • Cancer biology
    • Chromatin structural biology
    • Chromatin-based therapeutic strategies
  • Unique—the innovative CLOuD9 System is only sold by SBI

The CLOuD9 Gene Expression Regulation Kit comes with CLOuD9 Inducer Reagent and the two lentivector plasmids needed for expressing CLOuD9’s CRISPR/Cas9 System components (Figure 1):

  • One lentivector plasmid expressing a null Staphylococcus aureus Cas9 protein fused to one of the protein dimerization partners—the ABI protein from the plant abscisic acid (ABA) signaling pathway
  • A second lentivector plasmid expressing a null S. pyogenes Cas9 protein fused to the other protein dimerization partner—the PYL1 protein from the plant ABA signaling pathway

Figure 1. CLOuD9 constructs.

References

1. Morgan, SL, et al. Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping. Nat Commun. 2017. Jul 13; 8:15993. PMCID: PMC5511349.