High efficiency, RNA-guided
- All-in-one Cas9/gRNA plasmids
- Injection-ready Cas9 mRNA
- Packaged Cas9 lentivirus
- Multiplex gRNA cloning kits
- Expert technical support
Lentiviral CRISPR/Cas9 Systems
In addition to our industry-leading, all-in-one Cas9 SmartNuclease and SmartNickase plasmid systems, SBI now offers validated CRISPR/Cas9 & gRNA constructs in powerful lentivector formats. SBI's Lenti-Cas9 SmartNuclease system is ideal for targeting cell types that are difficult to transfect with plasmids, effectively expanding the range of target cells amenable for CRISPR/Cas9-based genome engineering. The Lenti-Cas9 system also provides an easy and efficient way to generate stable Cas9 editing cell lines. SBI offers lentiviral constructs in two formats:
1.) An "all-in-one" format expressing Cas9 and gRNA from a single vector
2.) A two vector system with separate Cas9 and gRNA expression vectors
All of the Cas9 lentivector constructs express human-codon optimized Cas9 wild-type nuclease or mutant nickase, while the gRNA cloning/expression lentivector constructs contain a pre-made tracrRNA scaffold with gRNA cloning sites driven by your choice of H1 or U6 Pol-III promoters for robust expression in a wide range of cells. The system is designed to accommodate flexible targeting of any genomic loci in the form of N20NGG; however, other gRNA formats (e.g. N17-18NGG) can be utilized as well.
SBI offers pre-packaged, ready-to-infect pseudoviral particles for expression of Cas9 wild-type nucleases and mutant nickases for generation of cell lines stably expressing Cas9. Pseudoviral particles have been packaged to exacting QC standards and comes with functional titer and in-house transduction data for each production lot of virus.
For those customers wishing to package any of the Lenti-Cas9 SmartNuclease lentivectors in their own lab, SBI offers the LentiStarter 2.0 kit, which contains all of the necessary reagents to produce high-quality virus and transduce target cells. The Kit is available as part of a bundled package with any Lenti-Cas9 vectors as well as a standalone item (SBI Cat #LV051A-1). Please refer to the LentiStarter 2.0 product webpage for details regarding how to use the LentiStarter 2.0 Kit.