High efficiency, RNA-guided
- All-in-one Cas9/gRNA plasmids
- Injection-ready Cas9 mRNA
- Packaged Cas9 lentivirus
- Multiplex gRNA cloning kits
- Expert technical support
Streamline genome-wide screens and generation of stable Cas9 cell lines
Combining the power of the AAVS1 safe harbor site with the ease and precision of CRISPR/Cas9, we offer both an AAVS1 Cas9-gene donor vector (Cat# CAS620A-1) and HEK293 cells with Cas9 already integrated and expressed from the AAVS1 site (Cat# CAS630A-1).
With our Cas9/HEK293 cells, you can go directly to transfection of your gRNAs and assessment of the biology - no need to spend time cloning Cas9 into the genome. And when you need Cas9 expression from other cell types, you can turn to our AAVS1 Cas9-gene donor vector and integrate Cas9 into the AAVS1 safe harbor site in the cells of your choice.
High-expression of the Cas9 gene at the AAVS1 locus in HEK293 cells, as determined by qPCR (left panel). A Surveyor Assay of high expressing clones #8 and #17 shows that Cas9 is functional - arrow corresponds to band indicative of Cas9-mediated cleavage event, which is not present in untransfected cells.