High efficiency, RNA-guided
- All-in-one Cas9/gRNA plasmids
- Injection-ready Cas9 mRNA
- Packaged Cas9 lentivirus
- Multiplex gRNA cloning kits
- Expert technical support
Background on How CRISPR/Cas9 Works
The recent discovery of the type II prokaryotic CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system, originally discovered in the bacterium Streptococcus pyogenes that works as a mechanism to defend against viruses and foreign DNA, has provided a revolutionary tool for targeted genome engineering with simple elegance.
The system uses a nuclease, CRISPR-associated (Cas9), that complexes with small RNAs as guides (gRNAs) to cleave DNA in a sequence-specific manner upstream of the protospacer adjacent motif (PAM) in any genomic location. The CRISPR-Cas9 system is comprised of separate guide RNAs known as the crRNA and tracrRNA. These two separate RNAs have been combined into a single RNA to enable site-specific mammalian genome cutting through the design of a short guide RNA. SBI has built a series of all-in-one CRISPR-Cas9 cloning vectors with different promoter options. These cloning vectors will express a human codon-optimized Cas9 nuclease as well as any gRNA sequence of your choice linked to the tracrRNA. Simply design the gRNA for the genome target DNA sequence and clone into the Cas9 SmartNuclease™ vector for efficient genome engineering. The PrecisionX™ Cas9 SmartNuclease™ Vector products are the subject of pending US and foreign patent applications.