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Powerful piggyBac Transposons
This webinar took place Thursday, July 28, 2016 at 10:00 AM PDT
Powerful PiggyBac: Applications for Genome Engineering and Inducible Expression
Learn how SBIs PiggyBac transposon products can help you with your genome engineering. See how our PiggyBac system can help you:
- Integrate large genes into your cells of interest. PiggyBac has enormous capacity for transgene delivery.
- Integrate and then excise sequences you insert into the genome.
- Create cumate-inducible constructs to control expression.
- Use PiggyBac technology for seamless, footprint-free CRISPR/Cas9 gene editing.
AAV-Cas9 SmartNuclease System
This webinar took place on Thursday, March 10, 2016 at 11:00 AM PDT
Powerful AAV Delivery for In Vivo Genome Engineering
Bringing together the versatile CRISPR/Cas9 genome editing system with powerful recombinant AAV (rAAV) technology, SBI's new AAV-Cas9 SmartNuclease vectors extend genome editing capabilities to cutting edge in vivo applications and difficult-to-transfect cell types. In this webinar, we will describe how to utilize recombinant adeno- associated viral technology with new Cas9 species for RNA-guided genome editing in human cells. We will also introduce a new safe harbor, site-specific knock-in system for more precise and controllable genome engineering, and we will review how this safe harbor system can be used to easily create stable, isogenic Cas9 cell lines for high-throughput screening.
XStamp: Program Exosomes with Specific Addresses
This webinar took place on Thursday, October 1, 2015 at 10:00 AM PDT
Program Exosomes with Specific Addresses -
Exosomes are extracellular nanoshuttles that facilitate communication between cells and can be engineered as therapeutic shuttles to deliver biological molecules or drugs to target disease cells. SBI has developed an exosome surface display system that enables desired protein sequences to be placed efficiently on the surfaces of engineered exosomes called the "XStamp" technology. The patented XStamp technology is based upon a C-terminal fusion of the C1C2 domain from MFG-E8. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes. The technology can be used to place cellular "addresses" on exosomes that send them to specific destinations for cargo delivery.
EV Shuttles: Next generation transfection system using exosomes
This webinar took place on Friday, July 10, 2015 at 10:00 AM PDT
Working with primary or other hard-to-transfect cells?
The Extracellular Vesicle (EV) Shuttle kit takes advantage of the natural ability of exosomes to be internalized and deliver functionally active biomolecules such as nucleic acids into recipient cells. Ready-to-use exosomes derived from human embryonic kidney cells (HEK 293) or murine dendritic cells (JAWS) can be transfected with nucleic acids including siRNA, miRNAs, mRNAs and plasmid DNA, and then added to a variety of hard-to-transfect cells for enhanced functional delivery.
In addition, this technology can be used to create stable cell lines in cells that are refractory to transfection by using EV shuttles loaded with the piggyBac transposon system. EV shuttles also offer significant advantages over virus-based transduction methodologies because they are non-viral, easy to handle and cost effective for delivery of RNA and DNA into target cells, with no virus packaging step required.
AAVanced™ Concentration Reagent rAAV Isolation System
This webinar took place on Thursday, April 23, 2015 at 10:00 AM PDT
Adeno-associated virus (AAV) is a single-strand DNA virus belonging to the Parvoviridae family. Recombinant adeno-associated virus (rAAV) vectors have been broadly used in gene therapy and genome engineering as an alternative to other viral gene delivery methods. However, traditional rAAV production methods require multiple steps, including cell lysis and CsCl2 ultra-high-speed density gradient centrifugation, chromatography, or binding to affinity matrix columns. These are difficult to setup, time-consuming, and require specialized equipment. SBI has developed an innovative rAAV concentration solution called AAVanced Concentration Reagent - a simple, one step rAAV concentration reagent for the isolation of rAAV particles from medium. AAVanced Concentration Reagent is based on a proven nanoparticle technology and is specifically optimized for the precipitation of most AAV serotypes. The rAAV virus produced using AAVanced Concentration Reagent has been tested successfully for in vitro and in vivo applications for multiple serotypes of AAV with no observed cytotoxic effects, which provides direct evidence for the utility of this reagent for demanding applications using rAAV. Join us for a live webinar to learn more about the AAVance Concentration Reagent on Thursday, April 23, 2015 at 10:00 am. After registering, you will receive a confirmation email containing information about joining the webinar.
New XPack Technology to Engineer Exosome Protein Cargo
This webinar took place on Thursday, April 2, 2015 at 10:00 AM PDT
The XPack technology developed by SBI allows researchers to insert any protein of interest into exosomes as cargo to be delivered to target cells. A suite of XPack reporter vectors enable tracking of exosome dynamics using fluorescence or luminescence assays, and XPack delivered proteins are shown to be functional within recipient cells. XPack reporters and other desired fusions are available in a lentivector backbone or as lentiviral particles ready for cell transduction, enabling users to generate stable cell lines constantly secreting exosomes packaged with the protein of interest.
Detect and Quantify Regulatory RNAs by qPCR
This webinar took place on Thursday, February 26, 2015 at 10:00 AM PDT
The genome is pervasively transcribed and large portion of the transcripts do not code for any proteins. These transcripts were initially thought to be junk but recent evidences suggest that these RNAs have regulatory potential. The potential functions of regulatory RNAs are now being explored as key layers of genome biology. Aberrant expression of these RNAs has been linked to cancer and other diseases. The RNAs holds promise in the areas of identifying novel therapies and for understanding complex cellular network signaling pathways. Very recently Scientists have found that enhancers are also actively transcribed and these RNAs are known as enhancer RNAs (eRNAs). Several eRNAs have been shown to have significant regulatory function in development and diseases. SBI has developed a regulatory RNA qPCR profiler that contains validated assays for the top regulatory RNAs involved in Cancer and Stem Cells. Join us for a live webinar to learn more regulatory RNA research on Thursday, February 26, 2015 at 10am. After registering, you will receive a confirmation email containing information about joining the webinar.
EEV: New Enhanced Episomal Vector System for Sustained Transgene Expression
This webinar took place on Thursday, February 5, 2015 at 10:00 AM PDT
SBI has developed a new, nonviral and non-integrating episomal vector system that allows for long term (at least 6 weeks), high-level transgene expression in cultured cells and in animal models. Register for this free webinar to learn more about the revolutionary EEV technology. Join us for a live webinar to learn more about how this new transgene expression technology can accelerate your research on Thursday, February 5, 2015 at 10am. After registering, you will receive a confirmation email containing information about joining the webinar.
Superior CRISPR/Cas9 Genome Editing using Multiplex gRNA Kits
This webinar took place on Wednesday, January 21, 2015 at 10:00 AM
Recently, the RNA-guided CRISPR/Cas9 system has been rapidly developed to serve as a new approach to alter the genome. A unique advantage of the CRISPR/Cas9 system is that Cas9 can be combined with two or more single guide RNAs (gRNAs) to achieve efficient multiplex genome targeting. To achieve uniform delivery of multiple gRNAs for CRISPR/Cas9-based genome engineering, SBI has developed a revolutionary and user-friendly system, the PrecisionX™ Multiplex gRNA Cloning Kit (Cat# CAS9-GRNA-KIT), which allows assembling multiple gRNAs from independent RNA polymerase III promoters into a single vector within 15 minutes by just one reaction. The new Multiplex gRNA Cloning Kit is also available bundled together with a Cas9 SmartNuclease or SmartNickase vector for added convenience (Cat# CS7xxA-KIT). At our upcoming webinar, we will demonstrate that each gRNA cloned with this system can be efficiently expressed and mediate multiplex gene editing, including advanced applications like tandem double-nicking to generate precise genomic deletions. The Multiplex gRNA Cloning Kit, compatible with SBI SmartNuclease and other popular Cas9/gRNA vectors (including the pX series), can be used to accelerate CRISPR/Cas9-based multiplex genome engineering in various cell types. Join us for a live webinar to learn more about how this new kit can speed up your research on Wednesday, January 21, 2015 at two times: 10am PST and again at 6pm PST. After registering, you will receive a confirmation email containing information about joining the webinar.
Engineer Exosome RNA Cargo for Target Cell Delivery
This webinar took place on Thursday, October 9, 2014 at 10:00 AM PDT
Exosomes contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery. Additionally, their natural function as cell to cell communication vehicles makes them attractive for use as therapeutic shuttles to deliver biological molecules or drugs to target disease cells. The RNA content varies, depending upon the cell from which the exosomes were secreted. The mechanism of how specific RNA sequences are selectively packaged into exosomes is an intensive area of investigation. SBI has previously pioneered the transfection of RNA and DNA directly into isolated exosomes using our unique Exo-Fect™ technology to enable exosomes to efficiently deliver RNA cargo to target cells. SBI has now developed a new technology for using cells to package specific miRNAs of choice into secreted exosomes - leveraging endogenous pathways to make cellular exosome factories. The new XMIR™ system uses a unique "XMotif" RNA sequence tag, which when fused to any miRNA or anti-miRNA traffics them to be packaged into exosomes by any transfected cell line.
How to use CRISPR/Cas9 Lentivirus and Lentivectors
This webinar took place on Thursday, October 2, 2014 at 10:00 AM PDT
RNA-guided, Cas9-mediated genome editing based on the Type II CRISPR/Cas system provides a new approach for modifying a target genome. With SBI's new Lenti-Cas9 SmartNuclease system, a broad variety of mammalian cells are now amenable for CRISPR/Cas9-based genome engineering. SBI's Lenti-Cas9 system provides an easy and efficient way to generate high titer Cas9 and gRNA lentiviruses which can be used to transduce difficult-to-transfect cell lines. In this webinar, we will show how easy it is to establish stable Cas9 editing cell lines, and review a simple and effective Lenti-Cas9 protocol to permanently knock-out a specific target gene in the human genome. Find out more at the upcoming Lenti-Cas9 Webinar, with two live presentations scheduled for Thursday, October 2, 2014 at 10:00am and 6:00pm PDT. Space is limited. Once registered, you will receive a confirmation email and a reminder email with the login details to attend the webinar.
Knockout MicroRNAs with TALE Nucleases and HR Vectors
This webinar took place on Wednesday, August 6, 2014 10:00 AM - 11:00 AM PDT
With SBI's new miR-KO System, bi-allelic microRNA ablation in the human genome is made easy and rapid by combining TALE Nucleases targeting a microRNA seed region with a homologous recombination donor vector to positively select knocked out cells. We will show how the miR-KO sets are designed and used, including sample data for knocking-out both of the human miR-21 loci.
Biomarker-X: Exosomes are the Future of Biomarkers in Medicine
This webinar took place on Tuesday, July 15, 2014 10:00 AM - 11:00 AM PDT
Watch On-demand Now
To discover accurate exosome biomarkers for any pathology, the best biomarkers are always derived from top-quality RNA or peptide libraries. SBI has developed kits that are specifically developed to generate high-quality NGS and Mass Spec libraries. The XRNA kit generates Illumina®NGS libraries from low-input exosome RNA samples for RNA-Seq. The XPEP Mass Spec kit is designed to provide a start-to-finish solution for making either exosome surface peptide libraries (aka "shaving") or creating complete peptide libraries from isolated exosomes that are compatible with direct loading onto most Mass Spec instruments. Find out Biomarker-X webinar scheduled for Tuesday July 15th, 2014 at 10:00am PDT.
• XRNA kit specifically developed for exosome RNA-Seq
• Works with low input amounts of exoRNA
• XPEP kit specially designed to make exosome surface or complete peptide libraries
• Load XPEP samples directly into MS/MS instrument
piggyBac Cell Line Engineering
This webinar took place on Thursday, February 27, 2014 10:00 AM - 11:00 AM PDT
Watch this recorded Webinar on-demand
The piggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a "cut and paste" mechanism. During transposition, the Super piggyBac transposase recognizes transposon-specific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The entire PB transposon can also be seamlessly excised using the new Excision-only piggyBac transposase. Find out how it all works in the upcoming piggyBac cell line engineering webinar scheduled for February 27th, 2014 at 10:00am PDT.
• Simple transgenesis with a single transfection using Super piggyBac transposase
• No cargo limit: integrate 10-100 kb easily
• Reversible, footprint-free excision with Excision-only piggyBac transposase
• Engineer model cell lines rapidly
Exo-Flow96: Immunopurify Exosomes from 96 Samples Simultaneously
This webinar took place on Thursday, December 12, 2013 10:00 AM - 11:00 AM PDT
Watch this Webinar
Exosomes are nanometer-sized membrane vesicles secreted by most cell types in vivo and in vitro and contain distinct subsets of RNAs and proteins depending upon the cell type from which they are secreted, making them useful for biomarker discovery and functional characterization. SBI has made it easy to obtain highly purified exosomes from samples using immunoaffinity magnetic beads. The new Exo-Flow96 kits enable the purification of exosomes from 96 samples all-at-once. Find out more by watching the on-demand Exo-Flow96 webinar.
Lentiviral technology webinar: overview and new bidirectional promoter formats
This webinar took place on Thursday, November 21, 2013 10:00 AM - 11:00 AM PDT
Watch this Webinar
Learn the basics of lentivectors and how to use this technology. Webinar topics to includes biosafety, choosing a lentivector, lentiviral packaging, transduction of target cells, and applications of lentiviral technology. SBI is also proudly introducing the bidirectional promoter lentivector formats that enable the co-expression of multiple transgenes simultaneously. Join us to find out how it all works at a webinar Thursday, November 14th at 10 AM PDT.
High Efficiency, Non-viral Episomal iPSC Generation
This webinar took place on Thursday, October 3, 2013 10:00 AM - 11:00 AM PDT
Watch this Recorded Webinar
Recently, the development of non-viral, non-integrating, plasmid-based reprogramming systems have gained popularity as an alternative to traditional retroviral-based reprogramming of cells. Notably, one of the systems, based on the Epstein-Barr Nuclear Antigen-1 (oriP/EBNA-1) has been proven to generate iPSCs very efficiently without the risk of transgenic sequences inserted into the target cell genome. Unlike traditional plasmid systems, the oriP/EBNA-1 system replicates in synchrony with the host genome by anchoring itself to the host chromatin and replicating during the cell cycle. After ~10-15 cell division cycles, the bulk of the episomal plasmid is lost, leading to the generation of reprogrammed cells free of any genomic integrations or genetic alterations. These transgene-free iPSCs have the capability to be utilized for a broad range of applications, including pre-clinical research and human gene therapy, thus further delivering on the promise of iPS cells. Join us to find out how it works on a webinar Thursday, October 3rd at 10 AM PDT.
New Tools for Mammalian Genome Engineering with the CRISPR-Cas9 System
This webinar took place on Thursday, September 26, 2013 10:00 AM - 11:00 AM PDT
Watch this Recorded Webinar
The Type II prokaryotic CRISPR-Cas system is a new class of tools for targeted genome engineering. The System uses small RNAs as guides to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genome locus, CRISPR-Cas system offers a simple, fast, and robust alternative to Zinc finger nuclease and TALEN platforms. At this webinar, we will describe how to design, express, and test human codon-optimized Cas9 protein and guide-RNAs for RNA-guided genome editing in human cells. Additionally, we will introduce the new Cas9 SmartNuclease transfection-ready mRNA kit, the T7 gRNA synthesis and mutant Cas9 Nickase vectors.
The PinPoint Targeted Integration System
This webinar took place on Thursday, August 15, 2013 11:00 AM - 12:00 PM PDT
Watch this Recorded Webinar
The PinPoint Targeted Integration system allows users to easily and efficiently create isogenic stable cell lines in mammalian and other cell types. Custom gene expression cassettes can be engineered into target genomes using the unique PinPoint integrase with site-specific control. This technology enables the generation of platform cell lines which can be used to routinely knock-in different transgenes and reporters at the same genetic locus in cells with the same genetic background. This level of targeting control allows for the study of phenotypic effects free from context and positional variations, which results in more accurate genotype to phenotype correlations. The PinPoint integrase can be used in combination with TALEN and Cas9 systems to provide high-throughput cell line engineering anywhere in the genome. Join us for a webinar this Thursday, August 15th at 11:00am PDT to learn more about this powerful new technology.
Genome Engineering 101: Cas9, TALENs and HR Targeting Vectors
This webinar took place on Friday, July 12, 2013 10:00 AM - 11:00 AM PDT
Watch Recorded Webinar
The Cas9/CRISPR and TALEN technologies have enabled researchers access to edit any locus within the genome in new and powerful ways through site-specific DNA cutting. In order to fully leverage the powerful nature of these genome engineering nucleases, one can use homology-directed recombination for targeted insertion at the sites of DNA cleavage. SBI has generated a suite of homologous recombination donor vectors which enable efficient editing at targeted genomic loci. The PrecisionX HR targeting vectors provide for simple HR arm additions that flank insulated marker expression cassettes with dual RFP or GFP and puro marker options, all with with built-in LoxP sites for later cassette removal. SBI also offers a specialized gene editing HR targeting vector that will allow for clean, single base pair modifications. Learn how to design and assemble accurate HR targeting vectors that work with Cas9/CRISPR and TALEN technologies. Perform simple selections on targeted cells to enrich for accurately engineered populations. SBI presents "Genome Engineering 101" in a recorded webinar that took place on Friday, July 12, 2013 at 10:00am PDT.
Mammalian Genome Engineering with the CRISPR-Cas9 System
Discover New RNA Biomarkers in Exosomes