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Transfection-ready Cas9 Protein

Rapid & Safe Cas9 Delivery

Highly efficient Cas9 Protein

  • Simplify delivery to cells and embryos
  • Reduce off-target events
  • Reduce the potential for immune response
  • Perform multiplex, high-throughput studies
  • Disease model generation of organisms and cell lines

Maximize CRISPR/Cas9 Performance, Safety with Transfection-ready Cas9 Protein

Taking the power of CRISPR/Cas9 technology to the next level, transfectable/electroporatable Cas9 protein delivers more efficient genome editing while reducing off-target events1–3. By removing plasmid delivery of Cas9 from the process, both off-target events from random plasmid integration and the potential for an immune response from bacterial plasmid sequences are avoided1,2. In addition, the more transient nature of Cas9 protein compared to plasmid or mRNA delivery further reduces off-target activity without decreasing on-target efficiency1,2. The end result is better, safer Cas9 activity for applications where off-target events need to be minimized, such as:

  • Genome engineering in embryos
  • Disease model generation of organisms and cell lines
  • In vitro transfection of cells
  • In vitro cleavage assays for functional gRNA screens

With transfectable/electroporatable Cas9 protein, you can:

  • Increase mutation efficiency
  • Reduce off-target events
  • Reduce the potential for immune response
  • Simplify delivery to cells and embryos
  • Perform multiplex, high-throughput studies
  • Conduct typical downstream functional assays

Get the Full System

Streamline genome editing with transfectable/electroporatable Cas9 protein—get Cas9 protein bundled into a kit (Cat #CAS400A-KIT) with the T7 gRNA Cloning and Production Kit.

References

1. Ramakrishna, S. et al. Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Genome Res. 24, 1020–1027 (2014).

2. Wang, L. et al. Large genomic fragment deletion and functional gene cassette knock-in via Cas9 protein mediated genome editing in one-cell rodent embryos. Sci. Rep. 5, 17517 (2015).

3. Chen, S., Lee, B., Lee, A. Y.-F., Modzelewski, A. J. & He, L. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes. J. Biol. Chem. (2016). doi:10.1074/jbc.M116.733154