Support
Contact SBI for technical support.
Locate vector sequences and read FAQs.
System Biosciences (SBI)
265 North Whisman Rd.
Mountain View, CA 94043
Direct: 650-968-2200
Toll Free: 888-266-5066
Fax: 650-968-2277
ExoQuick Frequently Asked Questions (FAQs)
Q: |
How do I decide which ExoQuick product to start with? ExoQuick or ExoQuick-TC?? | ||||||||||||||||||||
|
A:
|
ExoQuick should be used with biofluids that are concentrated in exosomes like serum, plasma, or ascites fluid. ExoQuick-TC should be used with biofluids that are dilute in exosomes like tissue culture (cell culture) media, urine, and CSF. | ||||||||||||||||||||
|
Q:
|
Can my biological fluid have been frozen before using ExoQuick? | ||||||||||||||||||||
|
A:
|
Frozen biofluids should be thawed on ice and then proceed with the regular ExoQuick protocol. | ||||||||||||||||||||
|
Q:
|
What are the typical yields of exosome, protein and RNAs from various biological fluids? | ||||||||||||||||||||
|
A:
|
See the values in the Table below. These are average yields.
|
||||||||||||||||||||
|
Q:
|
When I used ExoQuick, a large insoluble pellet was obtained and now it is difficult to work with. What should I do? | ||||||||||||||||||||
|
A:
|
Typically this happens if starting with plasma or serum. With plasma, there are a lot of fibrins and fibrinogens that also get precipitated with ExoQuick. We recommend adding the Thromboplastin D protocol before adding the ExoQuick, per the steps in the user manual. For serum, this typically occurs if you have incubated the ExoQuick with the serum for too long, or if the centrifugation step was too long. See the troubleshooting table below.
|
||||||||||||||||||||
|
Q:
|
What should I resuspend the precipitated exosomes with? | ||||||||||||||||||||
|
A:
|
This depends on what you want to do with the exosomes. Follow the guidelines here:
|
||||||||||||||||||||