Direct Conversion of Fibroblasts to Neurons

SBI's TD clone collection includes the mouse factors known to directly convert fibroblasts to neurons (Ascl1, Brn2, Myt1l, Olig2, and Zic1) as published in Wernig et al., Nature 2010. These vectors are expression-verified and functionally validated to convert mouse embryonic fibroblasts (MEFs) and tail fibroblasts directly to "Induced Neurons".

Direct Reprogramming: MEF Induced Neuronal Cells

Expression analysis and functional validation of the transdifferentiation factors Ascl1, Brn2, Myt1l, Olig2, and Zic1. Robust transcripts can be detected by RT-PCR and MEF induced neuronal cells using display predicted, complex neuronal morphology and express Tuj1 12 days after infection with five transcription factor lentiviruses.