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MicroRNA Reprogramming

Induce pluripotent stem cells using a single microRNA cluster

Use lentivirus, PiggyBac or minicircle DNA

  • miR-302bcad/367 cluster
  • Lentivector features GFP+Puro
  • Pre-packaged lentivirus ready to transduce
  • Minicircle vector co-expresses GFP
  • Pre-made minicircle DNA ready to transfect

miR-302bcad/367 PiggyBac Transposon vectors

PiggyBac transposon vectors enable integration through transfection. Choose from constitutive miR-302/367 expression (cat# PB-miR302) or inducible miR-302/367 expression via the cumate switch (cat# PBQM-miR302). After iPSC generation, the PiggyBac transposon can be removed, footprint-free from the genome by re-expressing the PiggyBac transpoase (cat# PB200PA-1).